Molecular detection of Giardia duodenalis in human and dogs
DOI:
https://doi.org/10.54174/rshdr762Keywords:
Giardia duodenalis, Molecular detectionAbstract
Study was used routine diagnosis & molecular detection of Giardia duodenalis in human and dog, 100 samples were taken from humans and 100 from dog. where 7% infected samples were found in humans and 13% infected samples were found in dog after examining it under a microscope, the flotation method was also use and stain with iodine (2-5%) lugols solution and Giemsa stain and use PCR maker genes TPI and SSU-rRna and sequencing genetic analysis. Concluded PCR is better technique for detecting giardia from lab culture.
Downloads
References
-Adam, R. D. (2001). Biology of Giardia lamblia. Clin Microbiol.
-AL-Khayat, F. A. A. M. (2015). Prevalence of Giardia lamblia in Asymptomatic Patients by Direct Examination and ELISA Methods: Fadia Abd Al-Muhsin AL-Khayat1, Farah Thamer Abdullah2 and Najwa Shhab Ahmed2. The Iraqi Journal of Veterinary Medicine, 39(1), 75-80.
-Amar, C. F., Dear, P. H., & McLauchlin, J. (2003). Detection and genotyping by real-time PCR/RFLP analyses of Giardia duodenalis from human faeces. Journal of Medical Microbiology, 52(8), 681-683.
-Basima, A. S. (2005). Study on the prevalence of intestinal parasites among children attending Al-Daura Health Centre-Baghdad. Iraqi National Journal of Nursing Specialties, 18(1), 53-57.
-Berrilli, F., Di Cave, D., De Liberato, C., Franco, A., Scaramozzino, P., & Orecchia, P. (2004). Genotype characterisation of Giardia duodenalis isolates from domestic and farm animals by SSU-rRNA gene sequencing. Veterinary Parasitology, 122(3), 193-199.
-Chen, L., Zhao, J., Li, N., Guo, Y., Feng, Y., Feng, Y., & Xiao, L. (2019). Genotypes and public health potential of Enterocytozoon bieneusi and Giardia duodenalis in crab-eating macaques. Parasites & vectors, 12(1), 1-11.
- Chiebao, D. P., Martins, C. M., Pena, H. F. D. J., Gabriel, F. H. D. L., Turazza, J., Soares, H., & Merlo, A. (2020). Epidemiological study of Giardia duodenalis infection in companion dogs from the metropolitan area of São Paulo Brazil. Zoonoses and Public Health, 67(7), 765-773.
-Dixon, B. R. (2021). Giardia duodenalis in humans and animals–transmission and disease. Research in veterinary science, 135, 283-289.
-Ebner, J., Koehler, A. V., Robertson, G., Bradbury, R. S., Jex, A. R., Haydon, S. R., ... & Gasser, R. B. (2015). Genetic analysis of Giardia and Cryptosporidium from people in Northern Australia using PCR-based tools. Infection, Genetics and Evolution, 36, 389-395.
-Erlandsen, S. L., & Meyer, E. A. (Eds.). (2013). Giardia and giardiasis: biology, pathogenesis, and epidemiology. Springer Science & Business Media.
-Ghieth, M.A., Kotb, M.A., Abu-Sarea, E.Y., El-Badry, A.A., 2016. Molecular detection of giardiasis among children at Cairo University Pediatrics Hospitals. J Parasit Dis. 40:1470-1474.
-Gómez-Muñoz, M. T., Cámara-Badenes, C., del Carmen Martínez-Herrero, M., Dea-Ayuela, M. A., Pérez-Gracia, M. T., Fernández-Barredo, S., ... & Fayer, R. (2012). Multilocus genotyping of Giardia duodenalis in lambs from Spain reveals a high heterogeneity. Research in Veterinary Science, 93(2), 836-842.
-Hadi, A. M., & Faraj, A. A. (2016). Prevalence of gastrointestinal Helminthes and protozoa among stray dogs in Baghdad. The Iraqi Journal of Veterinary Medicine, 40(1), 1-4.
-Heyworth, M. F. (2016). Giardia duodenalis genetic assemblages and hosts. Parasite, 23.
-Ibrahim, A. Q. (2012). Prevalence of Entamoeba histolytica and Giardia lamblia in Children in Kadhmiyah Hospital. The Iraqi Journal of Veterinary Medicine, 36(1), 32-36.
-Kawan, M. H. (2004). Epidemiological study of infection with intestinal protozoa: Cryptosporidium & Giardia & Eimeria in calves in Baghdad. The Iraqi Journal of Veterinary Medicine, 28(1), 122-133.
-Kraft, M. R., Klotz, C., Bücker, R., Schulzke, J. D., & Aebischer, T. (2017). Giardia's epithelial cell interaction in vitro: mimicking asymptomatic infection?. Frontiers in cellular and infection microbiology, 7, 421.
-Kuthyar, S., Kowalewski, M. M., Seabolt, M., Roellig, D. M., & Gillespie, T. R. (2022). Molecular characterization of Giardia duodenalis and evidence for cross‐species transmission in Northern Argentina. Transboundary and emerging diseases, 69(4), 2209-2218.
-Makawi, Z. A., & Al-Zubaidi, M. T. S. (2017). Parasitic contamination of drinking water and its prevalence among handlers and sheep. Iraqi J. of Sci, 41(2), 7-14.
-Monis, P. T., Mayrhofer, G., Andrews, R. H., Homan, W. L., Limper, L., & Ey, P. L. (1996). Molecular genetic analysis of Giardia intestinalis isolates at the glutamate dehydrogenase locus. Parasitology, 112(1), 1-12.
-Puebla, L. J., Núñez, F. A., Fernández, Y. A., Fraga, J., Rivero, L. R., Millán, I. A., ... & Silva, I. M. (2014). Correlation of Giardia duodenalis assemblages with clinical and epidemiological data in Cuban children. Infection, Genetics and Evolution, 23, 7-12.
-Read, C. M., Monis, P. T., & Thompson, R. A. (2004). Discrimination of all genotypes of Giardia duodenalis at the glutamate dehydrogenase locus using PCR-RFLP. Infection, genetics and evolution, 4(2), 125-130.
-Raof, S. A. W. (2011). Prevalence of Blastocystis hominis and Giardia lamblia parasites in patients of four regions in East–South Baghdad. The Iraqi Journal of Veterinary Medicine, 35(2), 74-84.
-Rumsey, P., & Waseem, M. (2018). Giardia Lamblia Enteritis.
-Waddood Al-mofti, A. (2005). The presence of protozoal cysts and helminthic ova on vegetables collected from Baghdad markets. Journal of the Faculty of Medicine Baghdad, 47(1), 89-91.
-Palmer, C. S., Traub, R. J., Robertson, I. D., Devlin, G., Rees, R., & Thompson, R. A. (2008). Determining the zoonotic significance of Giardia and Cryptosporidium in Australian dogs and cats. Veterinary parasitology, 154(1-2), 142-147.
-Piekara-Stępińska, A., Piekarska, J., Gorczykowski, M., & Bania, J. (2021). Genotypes of Giardia duodenalis in household dogs and cats from Poland. Acta Parasitologica, 66, 428-435.
-Robertson, I. D., & Thompson, R. C. (2002). Enteric parasitic zoonoses of domesticated dogs and cats. Microbes and Infection, 4(8), 867-873.
-Yu, F., Amer, S., Qi, M., Wang, R., Wang, Y., Zhang, S., ... & Zhang, L. (2019). Multilocus genotyping of Giardia duodenalis isolated from patients in Egypt. Acta tropica, 196, 66-71.
Downloads
Published
Issue
Section
License
Copyright (c) 2026 Fatima Khalid Al-khalidi
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
1.png){kind=spacer}
