Molecular detection of Salmonella bacteria in laying hens using Real-Time PCR and its impact on productive traits
DOI:
https://doi.org/10.54174/6wmrx052Keywords:
Salmonella spp-InvA gene-Real-Time PCR-Layer chickens-Egg qualityAbstract
This study aimed to evaluate the prevalence of Salmonella spp. Within the laying hen flocks, using a Real-time PCR reaction that affects the invA gene, in addition to studying the morphological and internal characteristics of deformed eggs compared to normal eggs, 100 blood samples were collected from the laying hens, and the PCR results showed that 55% of the flock were infected with Salmonella bacteria, indicating the spread of the disease. Also, 100 egg samples were analyzed, including 75 deformed eggs and 25 normal eggs. Its external and internal characteristics were analyzed, and the results were presented using descriptive statistics and t-test analysis. The analysis showed that the differences between deformed and normal eggs were statistically significant with respect to egg length, egg width, shell thickness, and yolk weight (P ≥ 0.001), while the differences in egg weight and albumen weight were not statistically significant (P > 0.05). These results highlight the importance of using molecular polymerase chain reaction for the rapid detection of infected cases and highlight the potential impact of infection on egg quality. The research concluded that there is a widespread prevalence of the disease in laying hen farms, emphasizing the need to conduct periodic infection and egg quality checks in order to improve
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