Expression of Glutamine Synthetase Leaf Isozyme (GS2) gene in eggplants infected with Eggplant mild leaf mottle virus that treated with Effective microorganism-1

Abstract

Eggplant has been recognized as an important host for a number of viruses, particularly a new emerging virus known as Eggplant mild leaf mottle virus (EMLMV). The purpose of the study was to determine how effective microorganism-1 (EM-1) affects the expression of the Glutamine Synthetase Leaf Isozyme (GS2) gene in eggplants infected with EMLMV. Effective microorganisms applied before (BE2), within (WE2) and after (AE2) artificial infection with EMLMV  and the treatments analyzed using RNA seq and bioinformatics. The symptoms of AE2-treated plants developed after 15 days, and when EM-1 was given, the bulk of the virus effect was reduced, and the injured plants recovered.  While the symptoms of BE2 were less severe than those of AE2, they did subside after seven days, and the plants recovered completely. In the case of WE2, there were no symptoms, and the plant appeared healthy. The mapping to the GS2 gene sequence revealed a higher number of assembled reads in the AE2 and BE2 treatments (30,748 and 29,061 respectively), whereas WE2 produced less mapped reads (18,136). The negative control CN received 10,653 reads, whereas the positive gained the fewest assembled reads (6,446 reads).