In vitro, effect of sucrose concentration and pH on MS medium contaminants
DOI:
https://doi.org/10.54174/a55nq645Keywords:
Tissue culture, explants, colonies, medium, fungiAbstract
A study was conducted in the laboratories of College of Agriculture and Marshlands, Department of Horticulture and Landscape Engineering, University of Thi Qar, Iraq. During the period from February to May 2024. Aims of a study to isolate and diagnose the most important fungi that infect agricultural media in tissue culture laboratories, and tested to effect of sucrose and pH in MS medium on the growth and development of contaminated fungi. The results confirmed that there is an effect of sucrose concentration added to the tissue culture medium and the acidity of the medium on the growth and development of fungal colonies, as 0 g.L-1 concentration did not record any fungal growth, while high concentrations of sucrose showed significant growth for all species under study. Also, a study showed that there is a significant effect of pH medium on the growth and development of fungal colonies depended the degree of response varies according to the type of fungus, and there were an interaction between the effect of pH medium with sucrose concentration , as the response of fungal species to this interaction differed towards inhibiting the growth of colonies despite the increase in the concentration of sucrose.
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