Effect of growth regulators on the morphological traits of three potato cultivars in vitro
DOI:
https://doi.org/10.54174/utjagr.v11i2.201Abstract
The experiments were conducted to study the effect of sub cultures (3 sub-cultures) of in vitro propagated plants of three cultivars of potato (Arnova, Riviera and Provento) and their interaction with Indole Acetic acid (IAA) at 0.0 , 1.0 mg L-1 and Benzyl Adenine ( BA) at 0.0, 0.5, 1.0 and 2.0 mg L-1 concentrations on morphological traits of vegetative (plant height, number of shoots, leaves , nodes and length of shoots) and roots (number of roots and their lengths) system. The cultures were incubated under temperature of 25 ± 2°C and illumination for 16 h day-1. The plants were transferred to the greenhouse and data were taken on the number of minitubers, their diameter and weight after 90 days. The results showed the effectiveness of IAA at 1 mg L-1 increasing in plant height (16.06 and 14.83 cm for Rivera and Provento cultivars, respectively), and in number of leaves and nodes (18.08 leaf plant-1 for Rivera and 16.92 nod plant -1 for Provento), and the number of roots and their length. (11.25 root plant-1 and 11.25 cm for Rivera and Arnova cultivar, respectively). The results also showed the effectiveness of the combination of IAA (1 mg L-1 ) + BA (0.5 mg L-1 ) increasing the number and length of shoots (6.50 shoot plant-1 and 4.83 cm for Provento and Rivera cultivar). A decrease in the number of roots was also observed with increasing concentrations of BA which added to the medium with or without of IAA. Results also recorded the highest average of diameter and weight of minitubers was 8.63 cm and 3.64 g for the mother plants of the Riviera and Provento cultivars, respectively, while the mother plants and in vitro propagated plants of three cultivars did not differ in the average number of minitubers.
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References
Abbas, M. A. F., & Ibrahim, M. A. (1996). The role of ethylene in the regulation of fruit ripening in the Hillawi date palm (Phoenix dactyliferaL). Journal of the Science of Food and Agriculture, 72(3), 306-308.
Ibrahim, M. A., Al-Taha, H., & Seheem, A. A. (2013). Effect of cytokinin type and concentration, and source of explant on shoot multiplication of pineapple plant (Ananas comosus' Queen') in vitro/Ucinek vrst in koncentracij citokininov ter vira stebelnih izseckov na in vitro razmnozevanje ananasa (Ananas comosus' Queen'). Acta agriculturae Slovenica, 101(1), 15.
Ibrahim, M. A. (2012). In vitro plant regeneration of local pummelo (Citrus grandis (L.) Osbeck.) via direct and indirect organogenesis. Genetics and Plant Physiology, 2(3-4), 187-191.
Ibrahim, M. A., & Daraj, I. A. (2015). Micropropagation of dahlia plants Dahlia variabilis Wild (Desf.). Effect of explant and plant growth regulators on shoot regeneration and growth. Advances in Agriculture & Botanics, 7(1), 1-6.
Ibrahim, M. A., Waheed, A. M., & Al-Taha, H. A. (2013). Plantlet regeneration from root segments of date palm tree (Phoenix dactylifera L. cv. Barhee) producing by in vitro culture. Advances in Agriculture & Botanics, 5(1), 45-50.
Abd Elhamid, M. I., Makboul, H. E., Sedik, M. Z., Ismail, I. M., & Ibrahim, M. A. (2010). Cloning, expression and antifungal activity of an endochitinase gene derived from barley (Hordeum vulgare). Research Journal of Agriculture and Biological Sciences, 6(3), 356-363.
Sharaf, A. N., Soliman, M. H., Ibrahim, M. A., Moustafa, S. A., & Hadi, A. (2007). Molecular identification and cloning of organophosphate degradation gene in some bacterial isolates. Arab Journal of Biotechnology, 10(2), 259-274.
Raza, T., Ibrahim, M., & Amjad, M. (1999). Seasonal changes in mineral nutrient and seed oil in Kinnow fruit (Citrus reticulata Blanco). Int. J. Agric. Biol, 4, 339-341.
Al-Sereh, E. A., Okash, A. N., & Ibrahim, M. A. (2020). THE EFFECT OF FOLIAR SPRAY WITH PRO. SOL FERTILIZER AND LICORICE EXTRACT ON SOME VEGETATIVE GROWTH INDICATORS FOR YOUNG POMEGRANATE (PUNICA GRANATUM L.) SEEDLINGS CV.'SALEMI'. Int. J. Agricult. Stat. Sci, 16(2), 739-74.
Abbas, M. F., & Ibrahim, M. A. (1998). Effect of pollen parent on the physiology of ripening of date palm fruit (Phoenix dactylifera L). Basrah J. Agric. Sci, 11(2), 31-37.
Ibrahim, M. A., Al-Taha, H. A., Saaid, Z. A., & Assist, R. PROPAGATION OF STRAWBERRY VIA IN VITRO ADVENTITIOUS SHOOT FORMATION.
Ibrahim, M. A., Metry, E. A., Moustafa, S. A., Tawfik, A. E., & Nasr El-Din, T. M. (2004). Morphological and molecular diagnosis of commercial potato cultivar infected with ralostonia solanacearum. Arab J Biotechnol, 8, 211-222.
Alnajjar, M. A., Alpresem, W. F., & Ibrahim, M. A. (2020). Effect of amino acid proline treatment on anatomical characteristics of leaves and roots of date palm seedlings Phoenix dactylifera L. developed under saline stress conditions. Plant Archives, 20(1), 755-760.
Ibrahim, M. A., Jasim, A. M., & Abbas, M. F. (2012). In vitro plant regeneration of Indian jujube (Ziziphus mauritiana Lamk.) cv. Zaytoni via indirect organogenesis. Acta Agric. Slov, 99(1), 65-67.
Ibrahim, M. A., Jasim, A. M., & Abbas, M. F. (2012). In vitro plant regeneration of Indian jujube (Ziziphus mauritiana Lamk.) cv. Zaytoni via indirect organogenesis. Acta Agric. Slov, 99(1), 65-67.
Ibrahim, M. (2022). Role of Endogenous and Exogenous Hormones in Bioactive Compounds Production in Medicinal Plants via In Vitro Culture Technique. Plant Hormones-Recent Advances, New Perspectives and Applications.
Al-Hamad, A. D. S., Alnajjar, M. A. H., & Ibrahim, M. A. (2021). The effect of alternating salinity of irrigation water and cultivation level on some soil properties and growth of date palm (Phoenix dactylifera L.) offshoots of Hillawi cultivar in clay soils. Int. J. Agricult. Stat. Sci. Vol, 17(1), 63-72.
Ibrahim, M. A., & Al-Sereh, E. A. (2019). Effect of foliar spray with potassium humate and green tea extract on some of the vegetative characteristics of guava (Psidium guajava L. cv. Local) seedlings. Plant Archives, 19(1), 404-408.
Ibrahim, M. A., Jerry, A. N., & Khalil, A. I. (2018). Effect of salt stress on some chemicals characteristics of callus for three cultivars of potato plant (Solanum tuberosum L.). Advances in Agriculture & Botanics, 10(2), 87-96.
Ibrahim, M. A., Jerry, A. N., & Khalil, A. I. (2017). Effect of plant growth regulators and sodium chloride on indirect organogenesis of three cultivars of potato plant (Solanum tuberosum L.) via in vitro culture. Advances in Agriculture & Botanics, 9(3), 103-110.
Safana, H. S., Ibrahim, M. A., & Abd, A. M. (2022). Impact of chitosan and benzyl adenine on shoot multiplication of kumquat plant (Citrus japonica Thumb.) in vitro. Int. J. Agricult. Stat. Sci. Vol, 18(1), 359-365.
Ibrahim, M., Ali, A. H., & Hashem, M. S. (2021). In vitro effects of cyanobacteria (Oscillatoria tenuis) extracellular products on date palm (Phoenix dactylifera L. cv.‘Barhee’) propagation. DYSONA-Applied Science, 2(1), 1-7.
Ibrahim, M. A., & Yassin, M. M. (2020). Direct shoot regeneration by in vitro culture of the gerbera (Gerbera jamesonii Bolus) capitulum explants. Plant Cell Biotechnol Mol Biol, 21, 1-9.
Salih, A. M., Alaradi, H. J., Ibrahim, M. A., & Alhello, A. A. (2019). Effect of some methods of planting in guava (Psidium guajava L.) seed germination. Plant Archives (09725210), 19(2).
Ibrahim, M. A., & Hamzah, H. A. (2019). Effect of the harvest date, storage period and package on the storability of date palm (phoenix dactylifera L. Cv. Taberzal) fruits. Plant Archives, 19(2), 226-231.
Taain, D. A., Ibrahim, M. A., & Al-Sareh, E. A. (2011). A study on the effect of postharvest calcium chloride treatments on the storability of mango fruits (Mangifera indica L.). Dirasat, 5, 37.
Abbas, M. F., Ibrahim, M. A., & Jasim, A. M. (2014). Micropropagation of Indian jujube (Ziziphus mauritiana Lam. cv. Zaytoni) through shoot tip culture. Advances in Agriculture & Botanics, 6(1), 11-15.
Ibrahim, M. A., Al-Taha, H., & AL-ALI, Z. T. (2017). Effect of casein hydrolysate and paclobutrazol on embryogenesis of date palm (Phoenix dactylifera L.) cv. Nersy by in vitro culture. Journal of Garmian University, 4(ICBS Conference), 395-404.
Ibrahim, M. A., Al-Aradi, H. J., & Muhsen, K. A. (2017). Effect of some growth regulators on proliferation of myrtle (Myrtus communis L. cv. Local) plant by in vitro culture. Advances in Agriculture & Botanics, 9(2), 97-101.
Ibrahim, M., & Yassin, M. M. (2021). Adventitious shoot proliferation from callus of Gerbera jamesonii young leaf explant. DYSONA-Applied Science, 2(4), 47-52.
SAFANA, H. S., ABD, A. M., & IBRAHIM, M. A. (2021). Study of genetic variations of In vitro micropropagation stages of Kumquat (Citrus japonica Thunb.). PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 292-299.
AL-JABIR, H. S. S., ABDULLA, A. A., & IBRAHIM, M. A. A STUDY OF SOME BIOACTIVE COMPONENTS IN WILD MALVA (Malva parviflora L.) PLANT IN DIFFERENT LOCATIONS OF BASRAH, SOUTHERN IRAQ.
Safana, H. S., Ibrahim, M. A. H. I., & Abd, A. K. M. (2022). Effect of NAA and Chitosan in rooting branches resulting from stem nodes plantation of Kumquat (Citrus japonica) in vitro. Journal of Kerbala for Agricultural Sciences, 9(1), 7-18.
Salih, A. M., Ibrahim, M. A., Al-Aradi, H. J., & Abass, M. H. (2020). The response of different date palm (Phoenix dactylifera L.) cultivars to callus induction and development by in vitro culture under salt stress. POLISH POLAR RESEARCH, 41(11), 23-41.
Ibrahim, M. A., Mohsen, A. T., Abdel-Mohsen, M. A., & Mostafa, A. S. (2014). Response of some Citrus rootstock seedlings to stimulating growth applications. Egypt. J. Hort, 41(2), 347-363.
AL-Hussaini, Z.A1 , Yousif , SH. A.1 and AL-Ajeely, S.A. 2015 . Effect of Different Medium on Callus Induction and Regeneration in Potato Cultivars. Int. J. Curr. Microbiol. App. Sci . 4(5): 856-865.
Al-Hussaini, Zainab Abdul-Jabbar Hussein. 2016 .The Employment of genetic variations in Potato (Solanum tuberosum L.) to improve salinity. PhD thesis. faculty of Agriculture. University of Kufa. Iraq
AL-Khazali, Sarah Raed Khalaf. 2016. Effect of growth regulators on callus induction and regeneration of the stock citrus Vulcamariana in vitro. Mse, College of Agriculture, University of Baghdad. Iraq.
Al-Salihi, Ali Abdul-Amir Mahdi. 2002. Sensitivity of potato Solanum tuberosum L. in vitro propagated to gamma rays. PhD thesis. faculty of Agriculture. Baghdad University. Iraq.
Al-Tikriti, Shatha Ayed Yousif. 2002. Evaluation and regeneration of salt-tolerant rice plants using different techniques. PhD thesis. faculty of Agriculture. Baghdad University. Iraq.
Chandra, S., Bandopadhyay, R., Kumar, V., Chandra, R., 2010: Acclimatization of tissue cultured plantlets: from laboratory to land. Biotech. Lett. 32, 1199-1205.
Delloloio, R. 2007. Cytokines determine Arabidopsis thaliana root meristem size by controlling cell differentiation, Curr. Biol. 17: 678–682.
George, E. F.; M. A. Hall and G. J. De Klerk .2008. Plant Propagation by Tissue Culture. Volume 1. The Background, 3rd Edition, Published by Springer, Dordrecht, The Netherlands.
Hartmann, H. J.; D. E. Kester; R. L. Geneve; and J. F. T. Davies.1997. Plant Propagation: Principles and Practices. 6th ed., Prentice – Hall Inc., New Jersey, USA.
Morais,T.P. ASMAR. S.A., Silva, H.F.J.,; LUZ., J.M.Q., and ; Berildo ., M. 2018. Application of tissue culture technique in Potato. Biosci. J., Uberlândia, vol (34), n0(4). p. 952-969,
Murashige, T. and Skoog, T. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant., 15: 473-479.
Nadia, Issaad and Adela, Elmi. 2018. Regenerative Biology of Plants. Micropropagation technique of Solanumtuberosum L. (Bartina and Kondor) potato cultivars under salt stress. Master Thesis. College of Natural and Life Sciences. University Brothers Mentouri and Constantine. Algeria.
Otroshy M, 2006. Utilization of tissue culture techniques in a seed potato tuber production scheme. 2-230
Rezadost, M. H.; M. M. Sohani,; A. Hatamzadeh and M. R. Mirzaii. 2013. In vitro regeneration of sour orange (Citrus aurantiumL.) via direct organogenesis. Plant Sci., 168: 583–591.
Sharifi, Muhammad Jaber Hassan. 2020. Effect of Light Sources, Nano-Iron and Zinc on Microtubers Production of Potato under Different CultureSystems. . PhD thesis. faculty of Agriculture. University of Kufa. Iraq.
Singh, B. and A. Kaur. 2011. Comparison of Agar and Gum Karaya as Gelling Agent for in vitro Regeneration of Rough Lemon (Citrus jambhiriLush.) Plantlets from Nodal Explants. J.Crop Sci . Biotech.14 (4):297-303.
Smulders. M. J. M. and • G. J. de Klerk.2011. Epigenetics in plant tissue culture. Plant Growth Regul. 63:137–146
Taiz, L. and E. Zeiger.(2010. Plant Physiology, 5th edition. Sinauer Associates Inc., Sunderland. 621 pages.
Teixeira, J., Hossain, M.M., Sharma, M., Dobránszki, J., Cardoso, J.C., Songjun, Z., 2017. Acclimatization of in vitro-derived Dendrobium. Hort. Plant J. 3, 110-124.
Wang, B., Y. Ma, Z. Zhang, Z. Wu, Y. Wu, Q. Wang, and M. Li. 2011. Potato viruses in China. Crop Protection 30 (9): 1117–1123.
Zahlout, Hanadi. 2006. plant hormones. Arab House for Publishing and Distribution, first edition, Beirut, Lebanon.
Saeed A. Mseer, Mahmoud S. Al-Ibrahimi and Falah H. Al-Miahy (2020). The effect of spraying with Nano fertilizers NPK and Gibberellin GA3 on the physical, chemical and physiological characteristics on the vegetative growth and fruit yield of Fig trees, black Diyala cultivar Ficus carica L. International Journal of Agricultural and Statistical Sciences. DocID: https:// connectjournals.com/03899.2020.16.1793
Maytham M. Kazem, Mahmoud S. Abdul Wahid and Ihssan J. Dhabib (2020). The effect of spraying with nutrient solution and organic fertilization on the vegetative and chemical characteristics of seedlings of domestic sour lemon (Citrus Limon L.). International Journal of Agricultural and Statistical Sciences. DocID: https://connectjournals.com/03899.2020.16.1787
Abdul-Wahid, M. S., Aqeel, H. A., & Rawaa, H. H. (2012). Effect of spray by Ascorbic and salicylic acid on some physiochemical properties of the local sour orange seedling citrus aurantium L. Thi-Qar University Journal for Agricultural Researches, 2(2222-5005).
Abdel-Wahed, M. S., & Mohsen, M. G. (2022). Effect of spraying with Ferti Mar and GROWX AMINO MIX on some chemical characteristics of Citrus aurantium seedlings Citrus aurantium L. University of Thi-Qar Journal of agricultural research, 11(2), 131-141.
Abdel-Wahed, M. S., & Hameed, L. A. (2022). Effect of glutathione and ascorbic acid on some physical characteristics of seedlings of grape plant Halawani cultivar Vitis vinifera L. University of Thi-Qar Journal of agricultural research, 11(2), 122-130.
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Abdul-wahid, M. S. (2019). Effect of Spray by Chelated Iron in physical and Chemical Characteristics of Iraqi Banana Musa Paradisica L. Var Sapientum (L.) Oktze Cultivation South Iraq. University of Thi-Qar Journal of agricultural research, 8(1).
Abdul-Wahid, M. S., & Abdulla, N. A. (2012). Effect of Iron Injection on some chemical Properties and yield of Date palm (phoenix dactylifera L.) cv kudrawe. Thi-Qar University Journal for Agricultural Researches, 1(2222-5005).
Jwar, A. S., & Abdul-Wahid, M. S. (2018). Effect of different pollen grain source and chitosan on some physiological and enzymatic characteristics of data palm Phoenix dactylifera L. fruits cv. Alshwethey. Basrah Journal For Date Palm Research, 17(1-2).
Abdul-wahid, M. S., & Jawar, A. (2019). Response of Some chemical, physical and productivity propertiesto date palmPhoenix dactylifera L. cultivation sites. University of Thi-Qar Journal of agricultural research, 8(1).
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-Jwar, A. S., & Neghamish, R. G. (2021). Effect of levels organic fertilizers and kinds of Bio-feritilizers in characterical of two classes of Okra plant (Abelmoschus esculentus L.) growning in the plastic house. University of Thi-Qar Journal of agricultural research, 10(1), 54-69.
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