Pathological study of pyocyanin that Extracted from Pseudomonas aeruginosa that isolated from human with otitis.
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https://doi.org/10.54174/ncmtb379Keywords:
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Abstract
This study 100 samples were taken from the ear of a person infected with otitis and the positive isolation rate of Pseudomonas aeruginosa was 26%, where samples were taken from males (45 cases), the positive was 9 infected samples (20%), and samples were taken from women (55 cases), the result was for 17 infected samples (30.9%). The isolation rate in adults was 34.28% and in children 6.6%, where the isolation was carried out using Cetrimide Media, MacConkey, and Blood Agar and was confirmed by biochemical tests and vitek 2system. Due to the importance of Pseudomonas aeruginosa, pyocyanin dye was extracted and It was injected into mice in different concentrations 50µg, 100µg, 150µg and 200µg and the mice were killed after two weeks of injection and the effects of histological pathology show a dense gathering of inflammatory cells in the organs (kidney, liver and lung) in all groups in addition to damage to the renal tubules.and in the lung of groups 150 and 200, severe fibrosis was observed as a result of cystic fibrosis due to pyocyanin.
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