Molecular characteristics of Pseudomonas aeruginosa isolated from human sources and chickens

Abstract

Samples were collected from different locations in Wasit Governorate, Iraq, 120human samplesTested from ear swabs, wounds, burns, feces and 153 chicken samplesP. aeruginosa isolates. Samples were striped on blood andMcConkey agarSuspect colonies are grown on chromogenic media and surrounded by citram. She was isolated The determination is made according to colony morphology, Gram staining, and conventional biochemistry Interactions and analysis profile of Index 20E (API 20E). A PCR examination confirmed the presence of P. aeruginosa in 11.35%. (273/31), 17.5% ( 120/21) from human samples, and 6.53% (153/10) from chickens.The polymerase chain reaction demonstrated that 100% of the isolates were P.Aeruginosa and all human and chicken isolates carry the exoA and exoT gene and the mexR gene, at a percentage (100%). And the study of histopathology on the internal organs (heart, lung, liver and intestine) of infected chickens.