Molecular characteristics of Pseudomonas aeruginosa isolated from human sources and chickens

Authors

  • Zainab Razzaq Zghair college of Veterinary Medicine
  • Waleed Khalid Mathree College of Veterinary Medicine, Zoonotic Unit, University of Baghdad, Iraq. https://orcid.org/0000-0001-7556-0043

DOI:

https://doi.org/10.54174/r4y2ab85

Keywords:

Pseudomonas aeruginosa, molecular study, Pseudomonas aeruginosa inchickens

Abstract

Samples were collected from different locations in Wasit Governorate, Iraq, 120human samplesTested from ear swabs, wounds, burns, feces and 153 chicken samplesP. aeruginosa isolates. Samples were striped on blood andMcConkey agarSuspect colonies are grown on chromogenic media and surrounded by citram. She was isolated The determination is made according to colony morphology, Gram staining, and conventional biochemistry Interactions and analysis profile of Index 20E (API 20E). A PCR examination confirmed the presence of P. aeruginosa in 11.35%. (273/31), 17.5% ( 120/21) from human samples, and 6.53% (153/10) from chickens.The polymerase chain reaction demonstrated that 100% of the isolates were P.Aeruginosa and all human and chicken isolates carry the exoA and exoT gene and the mexR gene, at a percentage (100%). And the study of histopathology on the internal organs (heart, lung, liver and intestine) of infected chickens.

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Published

2025-01-06

Issue

Section

Articles

How to Cite

Zghair, Z. R., & Waleed Khalid Mathree, W. K. M. (2025). Molecular characteristics of Pseudomonas aeruginosa isolated from human sources and chickens. University of Thi-Qar Journal of Agricultural Research, 14(1), 62-72. https://doi.org/10.54174/r4y2ab85