Synergistic Effect of Capparis Spinosa Fruits Extract in Comparison with Ciprofloxacin Against Resistant E. Coli O157:H7

Authors

  • Ali H. Saliem
  • Reham Najem Abdulridha

DOI:

https://doi.org/10.54174/utjagr.v11i2.205

Abstract

This study was conducted to evaluate the synergistic effect between Capparis Spinosa fruits methanolic extract and ciprofloxacin against resistant E. Coli O157:H7, in varying concentration. This experiment was carried out through the ultrasonic alcoholic extraction of C. Spinosa fruits, and an extraction ratio of 24% was obtained. The extract showed pronounced concentration dependent antibacterial activity. The susceptibility study revealed that E. Coli O157:H7 was sensitive to C. Spinosa fruits. The findings of the present study indicate that the use of pronounced C. Spinosa fruits extract may have the perfect to be choice in clinical control. The concentration of C. spinosa fruits extract increase the activity of inhibition, increase as well when used in combination with ciprofloxacin (half concentration from each other). In result appeared synergism effect between plant extract and ciprofloxacin and may make bacteria lost their resistance to antibiotics by this synergism phenomenon. The result of MIC (6400 µg/ ml), MBC (12800 µg/ ml) and for C. spinose fruits extract while ciprofloxacin MIC (12.5 µg/ ml) and MBC (25 µg/ ml). MICs of CIP/ C. spinosa fruits extract combinations against E. coli O157:H7 isolate was 1.562/ 1600 μg/ml. Value of minimum bactericidal concentration (MBC) of the synergistic combinations  (CIP/ C. spinosa fruits extract) was 3.124/ 3200 μg/ml. According to obtained results fractional inhibitory concentration (FIC) value of (CIP/ C. spinosa fruits extract 1.562/ 1600 μg/ml) was (0.375) less than 0.5 indicates synergistic effect of interaction.

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References

Abbas, M. A. F., & Ibrahim, M. A. (1996). The role of ethylene in the regulation of fruit ripening in the Hillawi date palm (Phoenix dactyliferaL). Journal of the Science of Food and Agriculture, 72(3), 306-308.‏

Ibrahim, M. A., Al-Taha, H., & Seheem, A. A. (2013). Effect of cytokinin type and concentration, and source of explant on shoot multiplication of pineapple plant (Ananas comosus' Queen') in vitro/Ucinek vrst in koncentracij citokininov ter vira stebelnih izseckov na in vitro razmnozevanje ananasa (Ananas comosus' Queen'). Acta agriculturae Slovenica, 101(1), 15.‏

Ibrahim, M. A. (2012). In vitro plant regeneration of local pummelo (Citrus grandis (L.) Osbeck.) via direct and indirect organogenesis. Genetics and Plant Physiology, 2(3-4), 187-191.‏

Ibrahim, M. A., & Daraj, I. A. (2015). Micropropagation of dahlia plants Dahlia variabilis Wild (Desf.). Effect of explant and plant growth regulators on shoot regeneration and growth. Advances in Agriculture & Botanics, 7(1), 1-6.‏

Ibrahim, M. A., Waheed, A. M., & Al-Taha, H. A. (2013). Plantlet regeneration from root segments of date palm tree (Phoenix dactylifera L. cv. Barhee) producing by in vitro culture. Advances in Agriculture & Botanics, 5(1), 45-50.‏

Abd Elhamid, M. I., Makboul, H. E., Sedik, M. Z., Ismail, I. M., & Ibrahim, M. A. (2010). Cloning, expression and antifungal activity of an endochitinase gene derived from barley (Hordeum vulgare). Research Journal of Agriculture and Biological Sciences, 6(3), 356-363.‏

Sharaf, A. N., Soliman, M. H., Ibrahim, M. A., Moustafa, S. A., & Hadi, A. (2007). Molecular identification and cloning of organophosphate degradation gene in some bacterial isolates. Arab Journal of Biotechnology, 10(2), 259-274.‏

Raza, T., Ibrahim, M., & Amjad, M. (1999). Seasonal changes in mineral nutrient and seed oil in Kinnow fruit (Citrus reticulata Blanco). Int. J. Agric. Biol, 4, 339-341.‏

Al-Sereh, E. A., Okash, A. N., & Ibrahim, M. A. (2020). THE EFFECT OF FOLIAR SPRAY WITH PRO. SOL FERTILIZER AND LICORICE EXTRACT ON SOME VEGETATIVE GROWTH INDICATORS FOR YOUNG POMEGRANATE (PUNICA GRANATUM L.) SEEDLINGS CV.'SALEMI'. Int. J. Agricult. Stat. Sci, 16(2), 739-74.‏

Abbas, M. F., & Ibrahim, M. A. (1998). Effect of pollen parent on the physiology of ripening of date palm fruit (Phoenix dactylifera L). Basrah J. Agric. Sci, 11(2), 31-37.‏

Ibrahim, M. A., Al-Taha, H. A., Saaid, Z. A., & Assist, R. PROPAGATION OF STRAWBERRY VIA IN VITRO ADVENTITIOUS SHOOT FORMATION.‏

Ibrahim, M. A., Metry, E. A., Moustafa, S. A., Tawfik, A. E., & Nasr El-Din, T. M. (2004). Morphological and molecular diagnosis of commercial potato cultivar infected with ralostonia solanacearum. Arab J Biotechnol, 8, 211-222.‏

Alnajjar, M. A., Alpresem, W. F., & Ibrahim, M. A. (2020). Effect of amino acid proline treatment on anatomical characteristics of leaves and roots of date palm seedlings Phoenix dactylifera L. developed under saline stress conditions. Plant Archives, 20(1), 755-760.‏

Ibrahim, M. A., Jasim, A. M., & Abbas, M. F. (2012). In vitro plant regeneration of Indian jujube (Ziziphus mauritiana Lamk.) cv. Zaytoni via indirect organogenesis. Acta Agric. Slov, 99(1), 65-67.‏

Ibrahim, M. A., Jasim, A. M., & Abbas, M. F. (2012). In vitro plant regeneration of Indian jujube (Ziziphus mauritiana Lamk.) cv. Zaytoni via indirect organogenesis. Acta Agric. Slov, 99(1), 65-67.‏

Ibrahim, M. (2022). Role of Endogenous and Exogenous Hormones in Bioactive Compounds Production in Medicinal Plants via In Vitro Culture Technique. Plant Hormones-Recent Advances, New Perspectives and Applications.‏

Al-Hamad, A. D. S., Alnajjar, M. A. H., & Ibrahim, M. A. (2021). The effect of alternating salinity of irrigation water and cultivation level on some soil properties and growth of date palm (Phoenix dactylifera L.) offshoots of Hillawi cultivar in clay soils. Int. J. Agricult. Stat. Sci. Vol, 17(1), 63-72.‏

Ibrahim, M. A., & Al-Sereh, E. A. (2019). Effect of foliar spray with potassium humate and green tea extract on some of the vegetative characteristics of guava (Psidium guajava L. cv. Local) seedlings. Plant Archives, 19(1), 404-408.‏

Ibrahim, M. A., Jerry, A. N., & Khalil, A. I. (2018). Effect of salt stress on some chemicals characteristics of callus for three cultivars of potato plant (Solanum tuberosum L.). Advances in Agriculture & Botanics, 10(2), 87-96.‏

Ibrahim, M. A., Jerry, A. N., & Khalil, A. I. (2017). Effect of plant growth regulators and sodium chloride on indirect organogenesis of three cultivars of potato plant (Solanum tuberosum L.) via in vitro culture. Advances in Agriculture & Botanics, 9(3), 103-110.‏

Safana, H. S., Ibrahim, M. A., & Abd, A. M. (2022). Impact of chitosan and benzyl adenine on shoot multiplication of kumquat plant (Citrus japonica Thumb.) in vitro. Int. J. Agricult. Stat. Sci. Vol, 18(1), 359-365.‏

Ibrahim, M., Ali, A. H., & Hashem, M. S. (2021). In vitro effects of cyanobacteria (Oscillatoria tenuis) extracellular products on date palm (Phoenix dactylifera L. cv.‘Barhee’) propagation. DYSONA-Applied Science, 2(1), 1-7.‏

Ibrahim, M. A., & Yassin, M. M. (2020). Direct shoot regeneration by in vitro culture of the gerbera (Gerbera jamesonii Bolus) capitulum explants. Plant Cell Biotechnol Mol Biol, 21, 1-9.‏

Salih, A. M., Alaradi, H. J., Ibrahim, M. A., & Alhello, A. A. (2019). Effect of some methods of planting in guava (Psidium guajava L.) seed germination. Plant Archives (09725210), 19(2).‏

Ibrahim, M. A., & Hamzah, H. A. (2019). Effect of the harvest date, storage period and package on the storability of date palm (phoenix dactylifera L. Cv. Taberzal) fruits. Plant Archives, 19(2), 226-231.‏

Taain, D. A., Ibrahim, M. A., & Al-Sareh, E. A. (2011). A study on the effect of postharvest calcium chloride treatments on the storability of mango fruits (Mangifera indica L.). Dirasat, 5, 37.‏

Abbas, M. F., Ibrahim, M. A., & Jasim, A. M. (2014). Micropropagation of Indian jujube (Ziziphus mauritiana Lam. cv. Zaytoni) through shoot tip culture. Advances in Agriculture & Botanics, 6(1), 11-15.‏

Ibrahim, M. A., Al-Taha, H., & AL-ALI, Z. T. (2017). Effect of casein hydrolysate and paclobutrazol on embryogenesis of date palm (Phoenix dactylifera L.) cv. Nersy by in vitro culture. Journal of Garmian University, 4(ICBS Conference), 395-404.‏

Ibrahim, M. A., Al-Aradi, H. J., & Muhsen, K. A. (2017). Effect of some growth regulators on proliferation of myrtle (Myrtus communis L. cv. Local) plant by in vitro culture. Advances in Agriculture & Botanics, 9(2), 97-101.‏

Ibrahim, M., & Yassin, M. M. (2021). Adventitious shoot proliferation from callus of Gerbera jamesonii young leaf explant. DYSONA-Applied Science, 2(4), 47-52.‏

SAFANA, H. S., ABD, A. M., & IBRAHIM, M. A. (2021). Study of genetic variations of In vitro micropropagation stages of Kumquat (Citrus japonica Thunb.). PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 292-299.‏

AL-JABIR, H. S. S., ABDULLA, A. A., & IBRAHIM, M. A. A STUDY OF SOME BIOACTIVE COMPONENTS IN WILD MALVA (Malva parviflora L.) PLANT IN DIFFERENT LOCATIONS OF BASRAH, SOUTHERN IRAQ.‏

Safana, H. S., Ibrahim, M. A. H. I., & Abd, A. K. M. (2022). Effect of NAA and Chitosan in rooting branches resulting from stem nodes plantation of Kumquat (Citrus japonica) in vitro. Journal of Kerbala for Agricultural Sciences, 9(1), 7-18.‏

Salih, A. M., Ibrahim, M. A., Al-Aradi, H. J., & Abass, M. H. (2020). The response of different date palm (Phoenix dactylifera L.) cultivars to callus induction and development by in vitro culture under salt stress. POLISH POLAR RESEARCH, 41(11), 23-41.‏

Ibrahim, M. A., Mohsen, A. T., Abdel-Mohsen, M. A., & Mostafa, A. S. (2014). Response of some Citrus rootstock seedlings to stimulating growth applications. Egypt. J. Hort, 41(2), 347-363.‏

Saeedi, P., Yazdanparast, M., Behzadi, E., Salmanian, A. H., Mousavi, S. L., Nazarian, S., & Amani, J. (2017). A review on strategies for decreasing E. coli O157: H7 risk in animals. Microbial pathogenesis, 103: 186-195.‏

Al-Taii, D. H. F., & Yousif, A. A. (2019). Effects of E. coli O157: H7 experimental infections on rabbits. The Iraqi Journal of Veterinary Medicine, 43(1), 34-42.‏

Yaseen, S. M., Saleh, A. M., & Al-Zubaidy, R. S. (2017). Contamination of the local produced broilers carcasses with Escherichia coli O157: H7 and its effect in public health in Diyala province. The Iraqi Journal of Veterinary Medicine, 41(2), 113-117.‏

Pal, M., Mulu, S., Tekle, M., Pintoo, S. V., & Prajapati, J. (2016). Bacterial contamination of dairy products. Beverage and food world, 43(9): 40-43.‏

Muraih, J. K., Arean, A. G., & Abdulabass, H. T. (2020). Phytochemical and antibacterial activity of Capparis spinosa roots extracts against some pathogenic bacteria. Ann Trop Med Public Health, 23(S10):‏ SP231010.

Ennacerie, F. Z., Filali, F. R., & Najia Moukrad, E. D. A. (2017). Antibacterial synergistic effect of extracts of the organs of capparis spinosa and in combination with antibiotics. International Journal of Advanced Research, 5(9): 1238-47.‏

Philcox, D. (2017). Capparaceae. In A revised handbook to the Flora of Ceylon. Routledge. 23-50.

Mohammed, G. J., Al-Jassani, M. J., & Hameed, I. H. (2016). Antibacterial, Antifungal Activity and Chemical analysis of Punica grantanum (Pomegranate peel) using GCMS and FTIR spectroscopy. International Journal of Pharmacognosy and Phytochemical Research, 8(3): 480-494.‏

Chedraoui, S., Abi-Rizk, A., El-Beyrouthy, M., Chalak, L., Ouaini, N., & Rajjou, L. (2017). Capparis spinosa L. in a systematic review: A xerophilous species of multi values and promising potentialities for agrosystems under the threat of global warming. Frontiers in Plant Science, 8: 1-8.

Nabavi, S. M., Russo, G. L., Tedesco, I., Daglia, M., Orhan, I. E., Nabavi, S. F., ... & Hajheydari, Z. (2018). Curcumin and melanoma: from chemistry to medicine. Nutrition and cancer, 70(2): 164-175.‏

Grimalt, M., Hernández, F., Legua, P., Almansa, M. S., & Amorós, A. (2018). Physicochemical composition and antioxidant activity of three Spanish caper (Capparis spinosa L.) fruit cultivars in three stages of development. Scientia horticulturae, 240: 509-515.‏

Mollica, A., Zengin, G., Locatelli, M., Stefanucci, A., Mocan, A., Macedonio, G., ... & Novellino, E. (2017). Anti-diabetic and anti-hyperlipidemic properties of Capparis spinosa L.: in vivo and in vitro evaluation of its nutraceutical potential. Journal of functional foods, 35: 32-42.

Shahrajabian, M. H., Sun, W., & Cheng, Q. (2021). Plant of the Millennium, Caper (Capparis spinosa L.), chemical composition and medicinal uses. Bulletin of the National Research Centre, 45(1): 1-9.‏

El-Ansari, M. A., Ibrahim, L. F., & Sharaf, M. (2018). Capparis spinosa L.: a natural source of pharmaceuticals. Egyptian Pharmaceutical Journal, 17(2): 61.‏

Anwar, F., Muhammad, G., Hussain, M. A., Zengin, G., Alkharfy, K. M., Ashraf, M., & Gilani, A. H. (2016). Capparis spinosa L.: A plant with high potential for development of functional foods and nutraceuticals/ pharmaceuticals. International Journal of Pharmacology, 12(3): 201-219.‏

Stefanucci, A., Zengin, G., Locatelli, M., Macedonio, G., Wang, C. K., Novellino, E., ... & Mollica, A. (2018). Impact of different geographical locations on varying profile of bioactives and associated functionalities of caper (Capparis spinosa L.). Food and chemical toxicology, 118: 181-189.‏

Rahnavard, R., & Razavi, N. (2017). A review on the medical effects of Capparis spinosa L. Advanced Herbal Medicine, 3(1): 44-53.‏

Mehrzadi, S., Mirzaei, R., Heydari, M., Sasani, M., Yaqoobvand, B., & Huseini, H. F. (2021). Efficacy and safety of a traditional herbal combination in patients with type II diabetes mellitus: a randomized controlled trial. Journal of Dietary Supplements, 18(1): 31-43.

Pandey, A. T., Pandey, I., Hachenberger, Y., Krause, B. C., Haidar, R., Laux, P., ... & Singh, A. V. (2020). Emerging paradigm against global antimicrobial resistance via bioprospecting of mushroom into novel nanotherapeutics development. Trends in Food Science & Technology, 106: 333-344.‏

Hematian, A., Nouri, M., & Dolatabad, S. S. (2020). Kashk with caper (Capparis spinosa L.) extract: quality during storage. Foods & Raw Materials, 8(2).‏

Mostafaloo, R., Asadi-Ghalhari, M., Izanloo, H., & Zayadi, A. (2020). Photocatalytic degradation of ciprofloxacin antibiotic from aqueous solution by BiFeO3 nanocomposites using response surface methodology. Global Journal of Environmental Science and Management, 6(2): 191-202.‏Zhang, G. F., Liu, X.,

Zhang, S., Pan, B., & Liu, M. L. (2018). Ciprofloxacin derivatives and their antibacterial activities. European journal of medicinal chemistry, 146: 599-612.‏

Davis, R.; Markham, A.; and Balfour, J. A. (1996). Ciprofloxacin drug, 51(6): 1019-1074.

Kawas, G., Marouf, M., Mansour, O., & Sakur, A. A. (2018). Analytical methods of ciprofloxacin and its combinations review. Research Journal of Pharmacy and Technology, 11(5): 2139-2148.‏

Pietsch, F., Bergman, J. M., Brandis, G., Marcusson, L. L., Zorzet, A., Huseby, D. L., & Hughes, D. (2016). Ciprofloxacin selects for RNA polymerase mutations with pleiotropic antibiotic resistance effects. Journal of Antimicrobial Chemotherapy, 72(1): 75-84.‏

Hooper, D. C., Wolfson, J. S., Ng, E. Y., & Swartz, M. N. (1987). Mechanisms of action of and resistance to ciprofloxacin. The American journal of medicine, 82(4A): 12-20.‏

Shybut, T. B., & Puckett, E. R. (2017). Triceps ruptures after fluoroquinolone antibiotics: a report of 2 cases. Sports Health, 9(5): 474-476.

Smith, N., Fackrell, R., & Henderson, E. (2016). Ciprofloxacin-associated bilateral iliopsoas tendon rupture: a case report. Age and Ageing, 45(5): 737-738.‏

Cozzani, E., Chinazzo, C., Burlando, M., Romagnoli, M., & Parodi, A. (2016). Ciprofloxacin as a trigger for bullous pemphigoid: the second case in the literature. American Journal of Therapeutics, 23(5): e1202-e1204.‏

Scavone, C., Mascolo, A., Ruggiero, R., Sportiello, L., Rafaniello, C., Berrino, L., & Capuano, A. (2020). Quinolones-Induced Musculoskeletal, Neurological, and Psychiatric ADRs: A Pharmacovigilance Study Based on Data from the Italian Spontaneous Reporting System. Frontiers in pharmacology, 11: 428.‏

Hajji, M., Jebali, H., Mrad, A., Blel, Y., Brahmi, N., Kheder, R., ... & Zouaghi, M. K. (2018). Nephrotoxicity of ciprofloxacin: five cases and a review of the literature. Drug Safety-Case Reports, 5(1): 1-5.

Arean, A. G., Ali, T. H., & Muraih, J. K. (2019). Extracted chemical compounds from Capparis spinosa leaves and their antibacterial activity on pathogenic bacteria. Journal of Pharmaceutical Sciences and Research, 11(2): 603-608.‏

Ghorbani, M., Aboonajmi, M., Ghorbani, J. M., & Arabhosseini, A. (2017). Effect of ultrasound extraction conditions on yield and antioxidant properties of the fennel seed (Foeniculum vulgare) extract.‏ J Food Sci Technol. 14(6): 63–73.

Quinn, P.J.; Carter, M.E.; Markey, B. and. Carter, G.R. (2004). Clinical Veterinary Microbiology. Mosby.Edinburgh, Lomdon, New York, Oxord and Philadephia. USA. pp:21-63.

Perez, C., Pauli, M. and Bezevque, P. (1990). An antibiotic assay by agar well diffusion method. Acta Biologiae Medicine Experimentalis, 15: 113-115.

CLSI (Clinical and Laboratory Standards Institute). (2018). Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated From Animals; Approved Standard 5th ed. CSLI Supplement VET08.Wayne, PA: Clinical and Laboratory Standards Institute USA, 33-53.

Veiga, A., Maria da Graça, T. T., Rossa, L. S., Mengarda, M., Stofella, N. C., Oliveira, L. J., and Murakami, F. S. (2019). Colorimetric microdilution assay: Validation of a standard method for determination of MIC, IC50%, and IC90% of antimicrobial compounds. Journal of microbiological methods, 162: 50-61.

Desouky, E. M., Shalaby, M. A., Gohar, M. K., & Gerges, M. A. (2020). Evaluation of antibacterial activity of silver nanoparticles against multidrug-resistant Gram negative bacilli clinical isolates from Zagazig University Hospitals. Microbes and Infectious Diseases, 1(1): 15-23.

Assadi, S., Shafiee, S. M., Erfani, M., & Akmali, M. (2021). Antioxidative and antidiabetic effects of Capparis spinosa fruit extract on high-fat diet and low-dose streptozotocin-induced type 2 diabetic rats. Biomedicine & Pharmacotherapy, 138: 111391.‏

Johnson, T. R., Case, C. L., Cappuccino, J. G., & Sherman, N. (2013). Great Adventures In The Microbiology Laboratory. Microbiology, 22: 175-176.‏

Anjum, A. (2015). Isolation of Shiga toxin producing Escherichia coli 0157: H7 from street food and raw vegetables in Dhaka City.

Disassa, N., Sibhat, B., Mengistu, S., Muktar, Y., & Belina, D. (2017). Prevalence and antimicrobial susceptibility pattern of E. coli O157: H7 isolated from traditionally marketed raw cow milk in and around Asosa town, western Ethiopia. Veterinary medicine international, Volume 2017, Article ID 7581531, 7 pages.

Rodriguez‐Souto, R. R., Garrido‐Maestu, A., Pastoriza‐Fontan, A., & Lozano‐Leon, A. (2017). Investigation and characterization of Shiga toxin‐producing Escherichia coli present in mussels from harvesting areas in Galician southern Rias (NW Spain). Journal of Food Safety, 37(4): e12367.

Novicki, T. J., Daly, J. A., Mottice, S. L., & Carroll, K. C. (2000). Comparison of sorbitol MacConkey agar and a two-step method which utilizes enzyme-linked immunosorbent assay toxin testing and a chromogenic agar to detect and isolate enterohemorrhagic Escherichia coli. Journal of Clinical Microbiology, 38(2): 547-551.‏

Yousif, A., & Al-Taii, D. (2014). Isolation and characterization of E. coli O157: H7 from human and animals. Mirror Res. Vet. Sci. Anim, 3(2): 11-18.‏

Al–Dawmy, F. A. A., & Yousif, A. A. (2013). Prevalence of E. coli O157: H7 in intestinal and urinary tract infection in children. Int. J. Adv. Res, 1(8): 111-120.‏

Zelyas, N., Poon, A., Patterson-Fortin, L., Johnson, R. P., Lee, W., & Chui, L. (2016). Assessment of commercial chromogenic solid media for the detection of non-O157 Shiga toxin-producing Escherichia coli (STEC). Diagnostic Microbiology and Infectious Disease, 85(3): 302-308.‏

Al-Saadi, Z. H., Tarish, A. H., & Saeed, E. A. (2018). Phenotypic detection and antibiotics resistance pattern of local serotype of E. coli O157: H7 from children with acute diarrhea in Hilla city/Iraq. Journal of Pharmaceutical Sciences and Research, 10(3): 604-609.‏

Paim, T. G. D. S., Cantarelli, V. V., & d'Azevedo, P. A. (2014). Performance of the Vitek 2 system software version 5.03 in the bacterial identification and antimicrobial susceptibility test: evaluation study of clinical and reference strains of Gram-positive cocci. Revista da Sociedade Brasileira de Medicina Tropical, 47 (3): 377-381.‏

Al-Humam, N. A. (2016). Special biochemical profiles of Escherichia coli strains isolated from humans and camels by the VITEK 2 automated system in Al-Ahsa, Saudi Arabia. Afric. J. Microb. Res. 10(22): 783-790.

AL-Azawi, A. H., Ghaima, K. K., & Salih, H. H. (2018). Phytochemical, antibacterial and antioxidant activities of Capparis spinosa L. Cultivated in iraq. Bioscience Research, 15(3): 2611-2618.‏

Hamad, L. R., Hussain, A. B., & Hassan, M. H. (2020). A Pharmacological Effects of Copparis spinosa Extracts on Pathogenic Escherichia Coli. International Journal of Pharmaceutical Research, 12(2): ‏0975-2366.

Oudah, S. K., Al-Salih, R. M., Gusar, S. H., & Roomi, A. B. (2019). Study of the role of polyphenolic extract of capparis spinosa L. leaves as acute toxicity and antibacterial agent. Plant Archives, 19(2): 3821-3829.‏

Hameed, A. T., Zaidan, D. H., & Dawd, S. M. (2021). The Phytochemical Constituent of Capparis Spinosa L. And Phenolic Activity on Pathogenic Bacteria and Blood Parameters.‏ systematic reviews in pharmacy, 12 (1): 1193-1198.

Al-Akedi, M. A. I., Al-Akedi, A. J., & Idris, K. (2012). Inhibitory activity of Capparis spinosa extracts against pathogenic microorganisms. Journal Of Education And Science, 25(63): 53-67.

Parekh, J. and Chanda, S. (2007). In vitro screening of antibacterial activity of aqueous and alcoholic extract of various Indian plant species against selected pathogens from Enterbacteriaceae. African Journal of microbiology research, 1(6): 92-99.‏

Elshikh, M., Ahmed, S., Funston, S., Dunlop, P., McGaw, M., Marchant, R. and Banat, I.M., (2016). Resazurin-based 96-well plate microdilution method for the determination of minimum inhibitory concentration of biosurfactants. Biotechnology letters, 38(6): 1015-1019.

Saeed A. Mseer, Mahmoud S. Al-Ibrahimi and Falah H. Al-Miahy (2020). The effect of spraying with Nano fertilizers NPK and Gibberellin GA3 on the physical, chemical and physiological characteristics on the vegetative growth and fruit yield of Fig trees, black Diyala cultivar Ficus carica L. International Journal of Agricultural and Statistical Sciences. DocID: https:// connectjournals.com/03899.2020.16.1793

Maytham M. Kazem, Mahmoud S. Abdul Wahid and Ihssan J. Dhabib (2020). The effect of spraying with nutrient solution and organic fertilization on the vegetative and chemical characteristics of seedlings of domestic sour lemon (Citrus Limon L.). International Journal of Agricultural and Statistical Sciences. DocID: https://connectjournals.com/03899.2020.16.1787

Abdul-Wahid, M. S., Aqeel, H. A., & Rawaa, H. H. (2012). Effect of spray by Ascorbic and salicylic acid on some physiochemical properties of the local sour orange seedling citrus aurantium L. Thi-Qar University Journal for Agricultural Researches, 2(2222-5005).‏

Abdel-Wahed, M. S., & Mohsen, M. G. (2022). Effect of spraying with Ferti Mar and GROWX AMINO MIX on some chemical characteristics of Citrus aurantium seedlings Citrus aurantium L. University of Thi-Qar Journal of agricultural research, 11(2), 131-141.‏

Abdel-Wahed, M. S., & Hameed, L. A. (2022). Effect of glutathione and ascorbic acid on some physical characteristics of seedlings of grape plant Halawani cultivar Vitis vinifera L. University of Thi-Qar Journal of agricultural research, 11(2), 122-130.‏

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Faiza K. Al-Rumaiydh, Falah H. Al-Miahy and Mahmud S. Ebd Alwahid (2020). The effect of Adding Nano NPK and Spraying by Salicylic Acid on some of the Major and Minor Metabolism Products of Pomegranate Seedlings, Cultivar Wonderful in Dhi-Qar Governorate. International Journal of Agricultural and Statistical Sciences. DocID: https://connectjournals.com/ 03899.2020.16.2051

Abdul-wahid, M. S. (2019). Effect of Spray by Chelated Iron in physical and Chemical Characteristics of Iraqi Banana Musa Paradisica L. Var Sapientum (L.) Oktze Cultivation South Iraq. University of Thi-Qar Journal of agricultural research, 8(1).‏

Abdul-Wahid, M. S., & Abdulla, N. A. (2012). Effect of Iron Injection on some chemical Properties and yield of Date palm (phoenix dactylifera L.) cv kudrawe. Thi-Qar University Journal for Agricultural Researches, 1(2222-5005).‏

Jwar, A. S., & Abdul-Wahid, M. S. (2018). Effect of different pollen grain source and chitosan on some physiological and enzymatic characteristics of data palm Phoenix dactylifera L. fruits cv. Alshwethey. Basrah Journal For Date Palm Research, 17(1-2).

Abdul-wahid, M. S., & Jawar, A. (2019). Response of Some chemical, physical and productivity propertiesto date palmPhoenix dactylifera L. cultivation sites. University of Thi-Qar Journal of agricultural research, 8(1).

Abdul-wahid, M. S. (2019). Effect of Spray by Chelated Iron in physical and Chemical Characteristics of Iraqi Banana Musa Paradisica L. Var Sapientum (L.) Oktze Cultivation South Iraq. University of Thi-Qar Journal of agricultural research, 8(1).

-Jwar, A. S., & Neghamish, R. G. (2021). Effect of levels organic fertilizers and kinds of Bio-feritilizers in characterical of two classes of Okra plant (Abelmoschus esculentus L.) growning in the plastic house. University of Thi-Qar Journal of agricultural research, 10(1), 54-69.‏

ISO 690

-Jwar, A. S., Wahed, M. S. A., & Ghani, S. T. (2021). Study the effect of foliar spraying with Dionyfer and Azomin on the vegetative growth indicators of the leaves of Henna plant (Lawsonia inermis L). University of Thi-Qar Journal of agricultural research, 10(1), 70-78.

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Published

2022-11-06

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Synergistic Effect of Capparis Spinosa Fruits Extract in Comparison with Ciprofloxacin Against Resistant E. Coli O157:H7 . (2022). Thi-Qar Journal of Agricultural Research, 11(2), 242-256. https://doi.org/10.54174/utjagr.v11i2.205