Genetic Variation of POU1F1 gene and Its Effect on Milk yields and Some Traits in Cyprus Goats
DOI:
https://doi.org/10.54174/smcxd313Keywords:
Goats, PCR-SSCP, Polymorphism, Transcription factor.Abstract
main goal of this study is to diagnose the polymorphisms of POU1F1 in Cyprus goats and its influence on some of their economic traits. In the current study goats belonging to the ruminant research station and living under the same condition. 75 goats were tested in this experiment. polymorphism was diagnosed using the polymerase chain reaction- single strand conformation polymorphism technique and Sanger sequencing to identify the variations in the animals. genotype and allele frequencies were done by POPGENE32. In this study, two alleles A and G with the frequencies 0.57 and 0.43 respectively, three genotypes GG, insA, and GA. The Frequencies of these genotypes were 0.21, 0.36, and 0.43 respectively. insA was significantly superior in the milk yield at a significance level of 0.05 in average daily milk, insA genotype showed a high value in this trait, 989, whereas GG and GA showed 875 and 859 g/day, respectively. On the other hand, some of the characteristics in milk yield demonstrated no differences between animals. From results, it is concluded that the POU1F1 influences milk yield in goats. Therefore, this gene could be considered a candidate to enhance the programs of farm animals and increase the income for these projects.
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