Melatonin, Niacin and Copper Supplementation in Cooling Tris Extender Enhances buffalo bulls semen characteristics
DOI:
https://doi.org/10.54174/88t6pg20Keywords:
Antioxidant, Cooling, SemenAbstract
This study explored the effect of melatonin, niacin and copper supplementation on sperm quality during different cooling periods of Iraqi buffalo bulls. Four adult bulls were used and divided equally into 4 groups. Melatonin (G1), Niacin (G2), Copper (G3) were added to tris extender and compared with the control group (C). Semen was collected for 7 weeks and cryopreserved during the cooling periods (0, 24, 48 and 72 days). The results of the cooling on individual motility show significant (P≤0.05) differences between the groups, with the G1, G2, and G3 outperforming the C in the periods. The percentage increased in the G1 and G2 and lower G3, while individual motility decreased in C. The percentage of live sperm also increased significantly (P≤0.05) in the G1, G2, and G3 during the different cooling periods, and decreased significantly in the C. G1 group exhibited a decreased abnormal sperm percentage when measured against the remaining groups. The results of the sperms plasma membrane integrity showed a significant (P≤0.05) superiority of the G1 during the periods of cooling, over the rest of the G2, G3 and C respectively. No significant differences in the acrosome integrity of sperm in the semen preserved at different cooling periods among the four groups. In conclusion, addition of melatonin, niacin, and copper to the semen diluent of Iraqi buffalo bulls significantly affected semen characteristics during different cooling periods, with melatonin being superior in improving some of these characteristics
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