Genetic polymorphism of FASN gene and its relationship to some body dimensions, growth, and fertility traits in Awassi sheep
DOI:
https://doi.org/10.54174/bbt3kx40Keywords:
FASN gene, Body dimensions, Fertility, Awassi sheepAbstract
Abstract: The study was conducted at the Sheep and Goat Research Station in Al-Shatra District, north of Thi-Qar, affiliated with the Thi-Qar Agriculture Directorate, and included 39 ewes of the Awassi sheep breed. The study aimed to analyze the genotyps of the FASN gene using the sequencing technique and to determine its relationship with growth traits, body dimensions, and fertility rate. DNA sequencing results in exons (39-38-37, 617 base pairs long) showed no mutations, while sequencing of exons (20-19-18) of 997 base pairs revealed a C>G mutation at position 562 within exon 20, resulting in an amino acid change from leucine to Valine. It is a missense mutation. Three genotypes have been identified (CC, CG, GG). The wild type (CC) was the most common (53.1%), with an allelic frequency of 0.69 for the C allele and 0.31 for the G allele. The results showed a significant effect of FASN gene genotypes on the rear height trait; CG ewes outperformed GG and CC ewes. A significant effect was also recorded on the birth weight of the fetus, with the GG combination being superior, without any significant differences being recorded in the fertility rate between the three combinations. The study concludes the importance of the FASN gene as a genetic indicator for improving productive performance in Awassi sheep by selecting genetic combinations associated with the best productive traits.
Downloads
References
Ajafar, M. H., Kadhim, A. H., & AL-Thuwaini, T. M. (2022a). The Reproductive Traits of Sheep and Their Influencing Factors. Reviews in Agricultural Science, 10, 82-89
Al-Mahdawi, Mazher Kadhim Kuaier (2011). Effect of using different protein levels in the diet and fattening period on body dimensions in Iraqi lambs. Diyala Journal of Agricultural Sciences, 3.
Al-Thuwaini, T. M. (2021). The relationship of hematological parameters with adaptation and reproduction in sheep; A review study. Iraqi Journal of Veterinary Sciences, 35(3), 575-580
Al-Thuwaini, T. M., & Kareem, Z. A. (2022). Dr. Novel missense variant L46Q of fatty acid synthase gene and fatty acids content in Awassi sheep. Acta Scientiarum. Animal Sciences, 44. . https://doi.org/10.4025/actascianimsci.v44i1.56271
Alzaydi, Marin Kadhim and Salim, Abdullah Hameed.(2021). Detection of the effect of PIT-1 gene genotypes on some productive traits in Holstein cattle. Thi-Qar Journal of Agricultural Research. Vol.10 (2).
Bicknell A.A., Cenik, C., Chua, H.N., Roth, F.P. and Moore, M.J. 2012. Introns in UTRs: Why We Should Stop Ignoring Them. Bio Essays. 34 (12): 1025-1034
Boligon,A.A., Baldi, F. and Albuquerque, L.G., 2011. Genetic parameters and relationships between growth traits and scrotal circumference measured at different ages in Nellore cattle. Genet Mol. Biol, 34:225-230.
Deacon, T. W. (2022). A degenerative process underlying hierarchic transitions in evolution. Biosystems 222, 104770.
Duncan, D.B. 1955. Multiple range and multiple F tests. Biometrics, 11, 1-41.
Esteves C., Livramento, K.G., Paiva, L.V., Peconick, A.P., Garcia, I.F., Garbossa, C.A. and Faria, P.B. 2019 The polymorphisms of genes associated with the profile of fatty acids of sheep. Arq. Bras. Med. Vet. Zootec., v.71, n.1, p.303- 313.
Gonzalez Berrios, C. L., Bowden, C. F., Saad, H. M., Van Campen, H., Bishop, J. V., Pinedo, P., Hansen, T. R., & Thomas, M. G. (2024). The relationship between FASN gene polymorphisms and fertility traits in Holstein cattle. Frontiers in Genetics, 15(6), 1-10.
Kaplanová K, Dufek A, Dračková E, Simeonovová J, Subrt J, Vrtková 1, Dvořák J. 2013. The association of CAPN1, CAST, SCD, and FASN polymorphisms with beef quality traits in commercial crossbred cattle in the Czech Republic. Czech J Anim Sci. 58: 489-496
Kawaguchi, F., Tsuchimura, M., Oyama, K., Matsuhashi, T., Maruyama, S., Mine, H., & Sasazaki, S. (2020). Effect of DNA markers on fertility traits of Japanese Black cattle for improving beef quantity and quality. Agriculture and Applied Biology Journal, 63(1), 9–20. https://doi.org/10.5194/aab-63-9-2020
Maamory, Y.and Al-Anbari,N . (2023). Relationship of FASN and ANXA9 genes polymorphism with Awassi ewes productivity (Doctoral dissertation, University of Baghdad, College of Agricultural Engineering Sciences, Department of Animal Production.
Naima, Ali Ghali, and Al-Anbari, Nasr Nouri (2022). The relationship of the genetic traits of the SCD and FASN genes with the productive performance of Awassi sheep. PhD Dissertation, University of Baghdad, College of Agricultural Engineering Sciences.
Singh, H.; Pannu, U.; Narula, H.K.; Chopra, A.; Naharwara, V.; Bhakar, S.K.(2016). Estimates of (co) variance components and genetic parameters of growth traits in Marwari sheep. J. Appl. Anim. Res. 2016, 44, 27–35.
SAS Statistical Analysis System, User's Guide. Statistical. (2018). Version 9.1th ed. SAS. Inst. Inc. Cary. N.C. USA.
Thbit, I.A.A., Abdulkareem, A.A. and Salim, A.H.(2021). Effect of CAPN3 gene genotypes on productive traits and carcass traits of broiler. University of Thi-Qar Journal of Agriculture Research, 10 (1),13-24.
Zhao, Q., Zeng, L., Wang, W., Yang, J., Ming, J., Zhao, Y., & Song, X. (2024). Identification of genetic polymorphisms and their expression and relationship with body size traits in Guizhou White goats (Capra hircus) with different breeds. Genes, 15(6), 656. . https://doi.org/10.3390/genes15060656.
Downloads
Published
Issue
Section
License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
1.png){kind=spacer}
