Physiological and Chemical Responses of Tissue-Cultured Barhi and Hilali Date of Foliar Amino Acid Application
DOI:
https://doi.org/10.54174/9g560035Keywords:
amino acids, variety, proteins, fruit set percentageAbstract
This study was conducted in one of the private orchards in Nasiriyah district, Thi- Qar Governorate, Iraq, during the growing season of 2023, with the aim of knowing the effect of spraying tissue-propagated Barhi and Hilali date palm trees cultivars with a mixture of some amino acids to improve fruit set, reduce the percentage of parthinocarpic fruits, and some chemical characteristics of fruits in the Hababok stage. The mixture of protein amino acids consisted of (L- Mithionine, L- Lysine, L- Glutamic, L- Arginine, and L- Tryptophan) at two concentrations (25 and 50) mg L-1 for each of them, in addition to their interactions and the comparison treatment. The results of the study showed that spraying with the mixture of amino acids led to a significant increase in each of the percentage of fruit set and concentrations of amino acids, total soluble proteins, and total soluble carbohydrates, and the concentration treatment (50 ml L-1) was significantly superior and achieved the highest averages of (72.83%), (6.95, 7.84, and 15.71) mg g-1, respectively. The concentration treatment (50 ml L-1) also achieved the lowest average of parthinocarpic fruits, reaching (24.41%). Regarding the cultivar effect, the Barhi cultivar significantly outperformed and achieved the highest averages for each of the percentage of fruit set and the fruit content of amino acids, total soluble proteins, and total soluble carbohydrates, reaching (68.98%), (5.42, 6.45, and 14.00) mg g-1, respectively, the Barhi cultivar also achieved the lowest average, with a significant difference, for the percentage of parthinocarpic fruit, reaching (30.58%), compared to the Hilali cultivar. Interaction coefficients between the study factors were significantly superior to the interaction treatment between the Barhi cultivar and amino acids at a concentration of (50 ml L-1), achieving the highest averages for each of the percentage of fruit set and its amino acid content, total soluble proteins and total soluble carbohydrates amounted to (74.68%) and (7.76, 8.63 and 18.47) mg g-1, respectively. This interaction treatment also achieved the lowest average and significant difference in the percentage of parthinocarpic fruits, reaching (21.83%) compared to the rest of the interaction treatments.
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Abdullah, A.A.; Faisal, H.A and Hzaa, A.Y.L. (2023). Response of date palm Phoenix dactylifera L. Hillawi cultivar to some amino acids. Journal of Global Innovations in Agricultural Sciences, 11(3), 341–346. https://doi.org/10.22194/JGIAS/23.1103
Al-Ibrahimi, J.H.M. and Al-Asadi, A.D.K. (2023). Effect of potassium , trace elements and cultivar on some physiological traits and sugars at the khalal stage of fruits of date palm ( Phoenix dactylifera L.) grown in Thi-Qar governorate. University of Thi-Qar journal of agricultural research, 12(1):112 – 129.
Ali-Dinar, H.; Mohammed, M., and Munir, M. (2021). Effects of Pollination Interventions, Plant Age and Source on Hormonal Patterns and Fruit Set of Date Palm (Phoenix dactylifera L.). Horticulturae, 7(11), 427. https://doi.org/10.3390/horticulturae7110427
Almutairi, K. F.; Saleh, A. A.; Ali, M. M.; Sas-Paszt, L.; Abada, H. S. and Mosa, W. F. A. (2022). Growth Performance of Guava Trees after the Exogenous Application of Amino Acids Glutamic Acid, Arginine, and Glycine. Horticulturae, 8(12), 1110. https://doi.org/10.3390/horticulturae8121110
Bavei, V.; Shiran, B.; Khodambashi, M. and Ranjbar, A. (2011). Protein electrophoretic profiles and physicochemical indicators of salinity tolerance in sorghum (Sorghum bicolor l.). African Journal of Biotechnology, 10(14), 2683–2697. https://doi.org/10.5897/ajb09.754
Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Anal Biochem, 72(1–2): 248–254. https://doi.org/10.1016/0003-2697(76)90527-3
Cohen, Y.; Korchinsky, R. and Tripler, E. (2004). Flower abnormalities cause abnormal fruit setting in tissue culture-propagated date palm (Phoenix dactylifera L.). The Journal of Horticultural Science and Biotechnology, 79(6), 1007–1013. https://doi.org/10.1080/14620316.2004.11511853
Darwesh, R. S. S. (2013). Improving growth of date palm plantlets grown under salt stress with yeast and amino acids applications. Annals of Agricultural Sciences, 58(2), 247– 256. https://doi.org/10.1016/j.aoas.2013.07.014
El-Badawy, H. (2019). Effect of Spraying Amino Acids and Micronutrients as Well as their Combination on Growth, Yield, Fruit Quality and Mineral Content of Canino Apricot Trees. Journal of Plant Production, 10(2), 125–132. https://doi.org/10.21608/jpp.2019.36242
El-Desouky, S. A.; Ismaeil, F. H.; Wanas, A. L.; Fathy, E.S. L. and AbdelAll, M. M. (2011). Effect of yeast extract, amino acids and citric acid on physioanatomical aspects and productivity of tomato plants grown in late summer season. Minufiya J. Agric. Res. 36(4): 859-884.
Fenn, M. A., and Giovannoni, J. J. (2021). Phytohormones in fruit development and maturation. The Plant Journal, 105(2), 446–458. https://doi.org/10.1111/tpj.15112
Ghilan, S. A. H. ; Suhaim, A. A. and Awad, K. A. M. (2024). Effect of spraying with some amino acids and plant growth regulators on fruit set percentage and some biochemical characteristics of tissue-propagated Barhi date palm (Phoenix dactylifera L.) fruits. Basra Journal of Date Palm Research, 23(1): 31-49.
Hussein, F. A. (2002). Description of some Iraqi date palm varieties. National Committee for Registration and Certification of Agricultural Varieties, National Program for the Propagation and Improvement of Date Palm Cultivation. Ministry of Agriculture - Republic of Iraq.
Ibrahim, A. B. A. (2008). The Date Palm Tree of Life. Arab Center for the Studies of Arid Zones and Dry Lands. Damascus- Syria: 390pp
Ibrahim, Z. F. (2014). The effect of spraying with proline and arginine on the growth and yield of eggplant ( Solanum melongena ) in protected cultivation. Master's thesis - College of Education for Pure Sciences - University of Diyala.
Kawade, K.; Tabeta, H.; Ferjani, A. and Hirai, M. Y. (2023). The Roles of Functional Amino Acids in Plant Growth and Development. Plant And Cell Physiology, 64(12), 1482–1493. https://doi.org/10.1093/pcp/pcad071
Kumar, R.; Khurana, A. and Sharma, A. K. (2014). Role of plant hormones and their interplay in development and ripening of fleshy fruits. Journal of Experimental Botany, 65(16), 4561–4575. https://doi.org/10.1093/jxb/eru277
Lee, Y. P., and Takahashi, T. (1966). An improved colorimetric determination of amino acids with the use of ninhydrin. Analytical Biochemistry, 14(1), 71–77. https://doi.org/10.1016/0003-2697(66)90057-1
Mirani, A. A.; Teo, C. H.; Abul-Soad, A. A.; Markhand, G. S.; Jatt, T., A., A.; Mirbahar, I and Solangi, N. (2019). Phenotypic Reversion of Somaclonal Variants Derived from Inflorescence of Date Palm (Phoenix dactylifera L.) in the Open Field Trials. Sarhad Journal of Agriculture, 35(3), 717.
Mirani, A. A.; Teo, C. H.; Markhand, G. S.; Abul-Soad, A. A. and Harikrishna, J. A. (2020). Detection of somaclonal variations in tissue cultured date palm (Phoenix dactylifera L.) using transposable element-based markers. Plant Cell, Tissue and Organ Culture, 141(1), 119–130. https://doi.org/10.1007/s11240-020-01772-y
Morales-Payan, J. P. (2015). Influence of foliar sprays of an amino acid formulation on fruit yield of “Edward” Mango. Acta Horticulturae, 1075, 157–159. https://doi.org/10.17660/ActaHortic.2015.1075.17
Mukherjee, A.; Gaurav, A. K.; Singh, S.; Yadav, S.; Bhowmick, S.; Abeysinghe, S. and Verma, J. P. (2022). The bioactive potential of phytohormones: A review. Biotechnology Reports, 35, e00748. https://doi.org/10.1016/j.btre.2022.e00748
Mukhina, M. T.; Lammas, M. E.; Korshunov, A. A. and Borovik, R. A. (2024). The influence of a complex of amino acids of plant origin with microelements on the yield and quality of apple trees. BIO Web of Conferences, 95, 01001. https://doi.org/10.1051/bioconf/20249501001
Omar, A.; Alobeed, R. S. and Al-saif, A. M. (2014). Effect of pollen source and area distribution on yield and fruit quality of “Khalas” date palm (Phoenix dactylifera, L.) under Saudi Arabia conditions. Acta Advances in Agricultural Sciences, 2(3): 7-13.
Pernisova, M.; Kuderova, A. and Hejatko, J. (2011). Cytokinin and Auxin Interactions in Plant Development: Metabolism, Signalling, Transport and Gene Expression. Current Protein and Peptide Science, 12(2), 137–147. https://doi.org/10.2174/1389211213488442037
Rahman, S.; Mehta, S. and Husen, A. (2024). Use of amino acids in plant growth, photosynthetic assimilation, and nutrient availability. In Biostimulants in Plant Protection and Performance (pp. 117–127). Elsevier. https://doi.org/10.1016/B978-0-443-15884- 1.00016-6
Salomón-Torres, R.; Krueger, R.; García-Vázquez, J. P.; Villa-Angulo, R.; Villa-Angulo, C.; Ortiz-Uribe, N.; Sol-Uribe, J. A. and Samaniego-Sandoval, L. (2021). Date palm pollen: Features, production, extraction and pollination methods. Agronomy, 11(3), 1–21. https://doi.org/10.3390/agronomy11030504
Salomon-Torres, R.; Ortiz-Uribe, N.; Villa-Angulo, R.; Villa-Angulo, C.; NorzagarayPlasencia, S. and García-Verdugo, C. D. (2017). Effect of pollenizers on production and fruit characteristics of date palm (Phoenix dactylifera L.) cultivar Medjool in Mexico. Turkish Journal of Agriculture and Forestry, 41(5), 338–347. https://doi.org/10.3906/tar1704-14
Taha, H.S.; Bekheet, S.A. and Saker, M.M.(2001). Factors affecting in vitro multiplication of date palm. Biologia plantarum 44(3): 431-433.
Watanabe, S.; Kojima, K.; Ide, Y. and Sasaki, S. (2000). Effects of Saline and Osmotic Stress on Proline and Sugar Accumulation in Populus euphratica in vitro Effects of saline and osmotic stress on proline and sugar accumulation in Populus euphratica in vitro. Plant Cell, Tissue and Organ Culture 63, 199–206 (2000). https://doi.org/10.1023/A:1010619503680
Yang, J.; Zhou, Y. and Jiang, Y. (2022). Amino Acids in Rice Grains and Their Regulation by Polyamines and Phytohormones. Plants, 11(12), 1581. https://doi.org/10.3390/plants11121581
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