The effect of adding lycopene on improving the characteristics of diluted and cooled semen of Arabi rams
DOI:
https://doi.org/10.54174/s5s9cn96Keywords:
Lycopene, characteristics, semen, Arabi rams, Computer- assisted semen analysis(CASA)Abstract
The experiment was conducted in the animal field of the College of Agriculture / University of Basrah / Karma Ali site for a period of three months from 10/15/2023 and in the forest 1/15/2024. Four Arabian rams were purchased from local markets in Basra Governorate, at ages ranging from 2.5 to 3 years and with similar weights. They were fed a diet consisting of a fodder mixture consisting of barley, bran, hay, and some mineral salts, clean water was provided to them inside the barn. The rams were trained in the process of collecting semen using the artificial vagina of rams in the animal field for a period of two weeks. After completing their training, semen was collected from them three times a month, and after the end of the training period, semen was collected three times a month. The study included adding different levels of lycopene (0,0.5,1,1.5 ) g/100 ml, and the tubes were closed tightly and placed in the refrigerator, and after the temperature stabilized at (5°C) for (0,24,48,72) hours, All characteristics of diluted and cooled semen were measured using a computer (CASA).
Downloads
References
Al-Sarray, M. A. R., Hobi, A. A. K. A. A. R., & Al-Ani, A. A. E. D. T. (2019). Effect of adding lycopene to the tris extender on Awassi rams semen quality preseveded at cooling and freezing storage. Plant Archives, 19(2), 3971–3976.
Amidi, F., Pazhohan, A., Shabani, M., Nashtaei, M. Khodarahmian, M., 2016. The role of antioxidants in sperm freezing: a review. Cell Tissue Bank. 17, 745-756. Doi: 10.1007/s10561-016-9566-5
Bintara, S., Widayati, D. T., Asmarawati, W., & Aji, R. N. (2023). The Effect of Lycopene Addition on the Semen Quality of Saanen Goats Stored at 5° C for 24 hours. Jurnal Ilmiah Peternakan Terpadu, 11(1), 34-40.
https://jurnal.fp.unila.ac.id/index.php/JIPT/article/view/6511
Chemineau, P., Cagnie, Y., Guerin, Y., Orgeur, P. and Vallet, J.C. (1991). Training manual on artificial insemination in sheep and goat. F.A.O. Animal Production and Health. pp. 83.
Choi, S.K. and J.S. Seo (2013). Lycopene supplementation suppresses oxidative stress induced by a high fat diet in gerbils. Nut. Res. & Prac., 7: 26–33.
https://synapse.koreamed.org/articles/1051297
He,Y.X., Wang,K., Zhao,X.X., Zhang,Y., Ma,Y.J. & Hu, J.J., (2016). Differential proteome association study of freeze-thaw damage in ram sperm. Cryobiology 72, 60-68. http://doi: 10.1016/j.cryobiol.2015.11.003.
Hossain, M. M. A., & Lee, S. S. (2019). Effect of freezing duration on antioxidant activity of ram semen. Journal of Animal and Veterinary Science., 20: 193-199.
Hyemin, C. & Dong G.L. 2015. Lycopene induces apoptosis in Candida albicans through reactive oxygen species production and mitochondrial dysfunction. Biochimie 115, 108-115. Doi: 10.1016/j.biochi.2015.05.009.
https://www.sciencedirect.com/science/article/abs/pii/S0300908415001431
Naviglio, D., F. Pizzolongo, L. Ferrara, A. Aragon and A. Santini(2008). Extraction of pure lycopene from industrial tomato by-products in water using a new high-pressure process. J. Sci. Food Agric., 88(14): 2414–2420.
Krishnamoorthy, G., K. Selvakumar, P. Elumalai, P. Venkataraman and J. Arunakaran (2011). Protective role of lycopene on polychlorinated biphenyls (aroclor 1254) induced adult rat sertoli cell dysfunction by increased oxidative stress and endocrine disruption. Biomed. Prev. Nutr., 1:116-125.
Souza, H.M. Arruda, L.C.P. Monteiro, M.M. I.H.A.V. Nery, R.A.J. Araújo Silva, A.S. Oliveira, Batista, A.M. & Guerra, M.M.P.2019. The in vitro investigation of lycopene effects on post-thawed ram sperm. South African Journal of Animal Science 2019, 49 (No. 5). DOI: 10.4314/sajas.v49i5.10
SPSS, Statistical Package for the Social Sciences . (2026). Quantitative Data Analysis with IBM SPSS version 26: A Guide for Social Scientists. New York: Routledge. ISBN 978-0-415-57918-6.
Syuhriatin. 2021. Effectiveness of Lycopene Antioxidant in Tomatoes (Lycopercisum esculentum) on Normality and Abnormality of Balinese Cow Spermatozoa by Swim up Method. Journal of Bionature, 22(1):9-14. DOI: 10.35580/bionature.v22i1.20604
Rosato, M.P., Centoducati, G., Santacroce, M.P. & Iaffaldano, N., (2012). Effects of lycopene on in vitro quality and lipid peroxidation in refrigerated and cryopreserved turkey spermatozoa. British Poultry Sci. 53, 545-552. https://doi: 10.1080/00071668.2012.716508.
Vincet,P.,Underward,S.L.,Dolbec,C.,Bouchard,V.,Kroetsch,T,and Blondin, P. (2012).
Bovine semen quality control in artificial insemination centers ..Anim.Reprod.,9:153-165.
Downloads
Published
Issue
Section
License
Copyright (c) 2025 Mashael Mohammed Abdulwahid, Falah Al-Asadi
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
1.png){kind=spacer}
