Expression of Newcastle disease virus genes and inflammatory biomarkers in Newcastle disease infected chicks
DOI:
https://doi.org/10.54174/kg11sm72Keywords:
Matrix gene, inflammatory biomarkers , RT-PCRAbstract
In poultry, the devastating effect of Newcastle disease (ND) has been addressed; however, the inflammatory response of infected birds has received less attention. Therefore, the study was designed to detect the ND virus and find changes in the gene expression of inflammatory biomarkers in birds infected naturally with ND using reverse transcription-PCR (RT-PCR). The expression of the matrix (M) gene of NDV and inflammatory biomarkers like creative reactive protein (CRP), interleukin 6, interleukin-1 beta (IL-6, IL-1β), and gamma interferon (IFN-γ) genes was analyzed. Despite the fact that flocks were vaccinated against NDV using the LaSota vaccine, various clinical signs were observed in many flocks, and 60 suspected and 30 control samples were collected from 3 to 5 weeks old. Blood, liver, and tracheal swaps were sampled from suspected and control birds. However, the infected birds showed respiratory, digestive, and nervous signs. Further, typical postmortem symptoms such as mottled spleen, periventricular gland-tip hemorrhages, and cecal hemorrhages were seen. The NDV infection was initially confirmed using the NDV rapid test, which showed positive results in 52 out of 60 suspected samples (86.66%). To confirm the infection with NDV, RT-PCR was utilized to examine the expression of the M gene in the samples and revealed that only 27 (51.92%) out of 52 samples were positive. Thereafter, the mean antibody levels of ND-infected birds were significantly lower than those of uninfected birds. In contrast, inflammatory biomarkers’ gene expression exhibited an increase in their mRNA levels, indicating that the birds were infected with a viral infection and that there is inflammation in the body. To sum up, data suggest that the M gene could be used as a marker for identifying NDV in infected birds. Similarly, the NDV infection leads to a decrease in AB titers, which is associated with an increase in the gene expression of inflammatory biomarkers in infected birds. Therefore, serological tests as well as the molecular approach should always be considered in epidemic regions
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Ahmed, A. I., and Odisho, S. M. (2018). Isolation identification and pathotyping of Newcastle disease viruses form naturally infected chickens In Iraqi Kurdistan region. The Iraqi Journal of Agricultural Sciences, 49(1), 132-141. DOI:10.36103/ijas.v49i1.216
Aliyu, M., Zohora, F. T., Anka, A. U., Ali, K., Maleknia, S., Saffarioun, M., and Azizi, G. (2022). Interleukin-6 cytokine: An overview of the immune regulation, immune dysregulation, and therapeutic approach. International Immunopharmacology, 111, 109130. DOI: 10.1016/j.intimp.2022.109130
Alsahami, A. A., Ideris, A., Omar, A., Ramanoon, S. Z., and Sadiq, M. B. (2018). Isolation, identification and molecular characterization of Newcastle disease viruses in vaccinated chickens from commercial farms in the Sultanate of Oman. International journal of veterinary science and medicine, 6(2), 248-252. DOI: 10.1016/j.ijvsm.2018.08.007
Amarasinghe, A., Abdul-Cader, M.S., Almatrouk, Z., Van der. Meer, F., Cork, S.C., Gomis, S., and Abdul-Careem, M.F. (2018). Induction of innate host responses characterized by production of interleukin (IL)-1β and recruitment of macrophages to the respiratory tract of chickens following infection with infectious bronchitis virus (IBV). Veterinary microbiology, 215:1–10. DOI: 10.1016/j.vetmic.2018.01.001
Awad, M., Mosad, S., and El-Kenawy, A. (2020). Molecular differentiation between velogenic isolates and lentogenic LaSota strain of Newcastle disease virus. Mansoura Veterinary Medical Journal, 21(4), 193-198. DOI: 10.21608/mvmj.2020.146340
Bhutia, L. D., Rajkhowa, T. K., Ravindran, R., Arya, R. S., Roychoudhury, P., Mandakini, R. K., and Singh, Y. D. (2017). Occurrence of Newcastle disease in poultry population of Mizoram, India. Indian Journal of Veterinary Pathology, 41(2), 151-154. DOI:10.5958/0973-970X.2017.00037.2
Cattoli, G., Susta, L., Terregino, C., and Brown, C. (2011). Newcastle disease: A review of field recognition and current methods of laboratory detection. Journal of Veterinary Diagnostic Investigation, 23(4): 637-656. DOI: 10.1177/1040638711407887.
Chhabra, R., Ball, C., Chantrey, J., and Ganapathy, K. (2018). Differential innate immune responses induced by classical and variant infectious bronchitis viruses in specific pathogen free chicks. Developmental & Comparative Immunology, 87, 16-23. DOIi: 10.1016/j.dci.2018.04.026
Chi, L., Li, Y., Stehno-Bittel, L., Gao, J., Morrison, D. C., Stechschulte, D. J., & Dileepan, K. N. (2001). Interleukin-6 production by endothelial cells via stimulation of protease-activated receptors is amplified by endotoxin and tumor necrosis factor-alpha. Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research, 21(4), 231–240. DOI: 10.1089/107999001750169871
Diel, D.G., da Silva, L.H.A., Liu, H., Wang, Z., Miller, P.J., and Afonso, C.L. (2012). Genetic diversity of avian paramyxovirus type 1: Proposal for a unified nomenclature and classification system of Newcastle disease virus genotypes. Infection, Genetics and Evolution, 12(8): 1770-1779. DOI: 10.1016/j.meegid.2012.07.012
Ecco, R., Susta, L., Afonso, C.L., Miller, P.J., and Brown, C. (2011). Neurological lesions in chickens experimentally infected with virulent Newcastle disease virus isolates. Avian Pathology, 40(2): 145-152. DOI: 10.1080/03079457.2010.544289
Fain, J.N. (2010). Release of inflammatory mediators by human adipose tissue is enhanced in obesity and primarily by the nonfat cells: a review. Mediators of Inflammation, 513948. DOI: 10.1155/2010/513948
Fensterl, V., and Sen, G.C. (2009). Interferons and viral infections. Biofactors 35: 14–20. DOI: 10.1002/biof.6
Getabalew, M., Alemneh, T., Akeberegn, D., Getahun, D., and Zewdie, D. (2019). Epidemiology, Diagnosis & Prevention of Newcastle disease in poultry. American Journal of Biomedical Science and Research, 16, 50-59. DOI: 10.34297/AJBSR.2019.03.000632
Hasan, A. R., Ali, M. H., Siddique, M. P., Rahman, M. M., and Islam, M. A. (2010). Clinical and laboratory diagnoses of newcastle and infectious bursal diseases of chickens. Bangladesh Journal of Veterinary Medicine, 8(2), 131-140. DOI:10.3329/bjvm.v8i2.11196
Kadhim, H. J., and Kuenzel, W. J. 2022. Interaction between the hypothalamo-pituitary-adrenal and thyroid axes during immobilization stress. Frontiers in Physiology, 13, 972171. DOI: 10.3389/fphys.2022.972171
Kadhim, H. J., Kang, S. W., and Kuenzel, W. J. 2019. Differential and temporal expression of corticotropin releasing hormone and its receptors in the nucleus of the hippocampal commissure and paraventricular nucleus during the stress response in chickens (Gallus gallus). Brain Research, 1714, 1–7. DOI: 10.1016/j.brainres.2019.02.018
Kadhim, H. J., Kang, S. W., and Kuenzel, W. J. 2021. Possible roles of brain derived neurotrophic factor and corticotropin releasing hormone neurons in the nucleus of hippocampal commissure functioning within the avian neuroendocrine regulation of stress. Stress (Amsterdam, Netherlands), 24(5), 590–601. DOI: 10.1080/10253890.2021.1929163
Kadhim, H. J., Kidd, M., Jr, Kang, S. W., and Kuenzel, W. J. 2020. Differential delayed responses of arginine vasotocin and its receptors in septo-hypothalamic brain structures and anterior pituitary that sustain hypothalamic-pituitary-adrenal (HPA) axis functions during acute stress. General and Comparative Endocrinology, 286, 113302. DOI: 10.1016/j.ygcen.2019.113302.
Kaiser P. (2010). Advances in avian immunology--prospects for disease control: a review. Avian pathology, 39(5), 309–324. DOI: 10.1080/03079457.2010.508777
Kim, K.S., Jung, H., Shin, I.K., Choi, B.R., and Kim, D.H. (2015). Induction of interleukin-1 beta (IL-1β) is a critical component of lung inflammation during influenza A (H1N1) virus infection. Journal of medical virology, 87:1104–1112. DOI: 10.1002/jmv.24138
Mao, Q., Ma, S., Schrickel, P. L., Zhao, P., Wang, J., Zhang, Y., ... and Wang, C. (2022). Review detection of Newcastle disease virus. Frontiers in Veterinary Science, 9, 936251. DOI: 10.3389/fvets.2022.936251
Millar, N.S., Emmerson, P.T. (1988). Molecular Cloning and Nucleotide Sequencing of Newcastle Disease Virus. In: Alexander, D.J. (eds) Newcastle Disease. Developments in Veterinary Virology, vol 8. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1759-3_5
Mitra, S., and Leonard, W. J. (2018). Biology of IL‐2 and its therapeutic modulation: Mechanisms and strategies. Journal of leukocyte biology, 103(4), 643-655. DOI: 10.1002/JLB.2RI0717-278R
Peiró, C., Lorenzo, Ó., Carraro, R., & Sánchez-Ferrer, C. F. (2017). IL-1β Inhibition in Cardiovascular Complications Associated to Diabetes Mellitus. Frontiers in pharmacology, 8, 363. DOI: 10.3389/fphar.2017.00363
Queiroz, M. A. F., Neves, P. F. M. D., Lima, S. S., Lopes, J. D. C., Torres, M. K. D. S., Vallinoto, I. M. V. C., Bichara, C. D. A., Dos Santos, E. F., de Brito, M. T. F. M., da Silva, A. L. S., Leite, M. M., da Costa, F. P., Viana, M. N. D. S. A., Rodrigues, F. B. B., de Sarges, K. M. L., Cantanhede, M. H. D., da Silva, R., Bichara, C. N. C., van den Berg, A. V. S., Veríssimo, A. O. L., Vallinoto, A. C. R. (2022). Cytokine Profiles Associated With Acute COVID-19 and Long COVID-19 Syndrome. Frontiers in cellular and infection microbiology, 12, 922422. DOI: 10.3389/fphar.2017.00363
Reda, A.M. and Jasim, N. S. (2022). Molecular Diagnosis of Newcastle Disease Virus by RT-RCR and Detection Fusion Gene Using Conventional PCR. Journal of Pharmaceutical Negative Results, 13 (03):983-944. DOI: 10.47750/pnr.2022.13.S03.144
Sawant, P., Verma, P., Subudhi, P., Chaturvedi, U., Singh, M., Kumar, R., and Tiwari, A. (2011). Immunomodulation of bivalent Newcastle disease DNA vaccine induced immune response by co-delivery of chicken IFN-γ and IL-4 genes. Veterinary immunology and immunopathology, 144: 36–44. DOI: 10.1016/j.vetimm.2011.07.006
Schmittgen, T. D., & Livak, K. J. (2008). Analyzing real-time PCR data by the comparative C(T) method. Nature protocols, 3(6), 1101–1108. DOI: 10.1038/nprot.2008.73
Schultz, U., & Chisari, F. V. (1999). Recombinant duck interferon gamma inhibits duck hepatitis B virus replication in primary hepatocytes. Journal of virology, 73(4), 3162–3168. DOI: 10.1128/JVI.73.4.3162-3168.1999
Selim, K., Adel, A., Eid, S., & Shahein, M. (2022). Development of real time reverse transcription loop-mediated isothermal amplification assay for rapid detection of genotype VII of Newcastle disease viruses. British poultry science, 63(6), 864–870. DOI: 10.1080/00071668.2022.2094219
Sen, A., Torab, A., Salam, S. M. A., Halder, B., and Alauddin, M. D. (2017). Comparative Study on Newcastle Disease and Infectious Bursal Disease in Chicken Submitted to Upazilla Veterinary Hospital, Bogra Sadar. Bangladesh Journal of Veterinary Science and Technology, 8(2).
Shabbir, M. Z., Zohari, S., Yaqub, T., Nazir, J., Shabbir, M. A., Mukhtar, N., Shafee, M., Sajid, M., Anees, M., Abbas, M., Khan, M. T., Ali, A. A., Ghafoor, A., Ahad, A., Channa, A. A., Anjum, A. A., Hussain, N., Ahmad, A., Goraya, M. U., Iqbal, Z., … Munir, M. (2013). Genetic diversity of Newcastle disease virus in Pakistan: a countrywide perspective. Virology journal, 10, 170. DOI: 10.1186/1743-422X-10-170.
Susta, L., Cornax, I., Diel, D. G., Garcia, S. C., Miller, P. J., Liu, X., Hu, S., Brown, C. C., & Afonso, C. L. (2013). Expression of interferon gamma by a highly virulent strain of Newcastle disease virus decreases its pathogenicity in chickens. Microbial pathogenesis, 61-62, 73–83. DOI: 10.1016/j.micpath.2013.05.009
Tanaka, T., Narazaki, M., & Kishimoto, T. (2014). IL-6 in inflammation, immunity, and disease. Cold Spring Harbor perspectives in biology, 6(10), a016295. DOI: 10.1101/cshperspect.a016295
Thi, H. T. H., & Hong, S. (2017). Inflammasome as a Therapeutic Target for Cancer Prevention and Treatment. Journal of cancer prevention, 22(2), 62–73. DOI: 10.15430/JCP.2017.22.2.62
Thomas, P.G., Dash, P., Aldridge, J.R. Jr., Ellebedy, A.H., Reynolds, C., Funk, A,J., Martin, W.J., Lamkanfi, M., Webby, R.J., Boyd, K.L., Doherty, P.C., and Kanneganti, T.D. (2009). The intracellular sensor NLRP3 mediates key innate and healing responses to influenza A virus via the regulation of caspase-1. Immunity, 30:566–575. DOI: 10.1016/j.immuni.2009.02.006
Wang, B., Zhu, J., Li, D., Wang, Y., Zhan, Y., Tan, L., Qiu, X., Sun, Y., Song, C., Meng, C., Ying, L., Xiang, M., Meng, G., & Ding, C. (2016). Newcastle disease virus infection induces activation of the NLRP3 inflammasome. Virology, 496, 90–96. DOI: 10.1016/j.virol.2016.05.023
Wang, Z., Liu, H., Xu, J., Bao, J., Zheng, D., Sun, C., Wei, R., Song, C., & Chen, J. (2006). Genotyping of Newcastle disease viruses isolated from 2002 to 2004 in China. Annals of the New York Academy of Sciences, 1081, 228–239. DOI: 10.1196/annals.1373.027
Worku, T., Dandecha, M., Shegu, D., Aliy, A., & Negessu, D. (2022). Isolation and Molecular Detection of Newcastle Disease Virus from Field Outbreaks in Chickens in Central Ethiopia. Veterinary medicine (Auckland, N.Z.), 13, 65–73. DOI: 10.2147/VMRR.S352727
Yeh, H. Y., Winslow, B. J., Junker, D. E., & Sharma, J. M. (1999). In vitro effects of recombinant chicken interferon-gamma on immune cells. Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research, 19(6), 687–691. DOI: 10.1089/107999099313848
Zheng, Z., Peng, F., Xu, B., Zhao, J., Liu, H., Peng, J., Li, Q., Jiang, C., Zhou, Y., Liu, S., Ye, C., Zhang, P., Xing, Y., Guo, H., & Tang, W. (2020). Risk factors of critical & mortal COVID-19 cases: A systematic literature review and meta-analysis. The Journal of infection, 81(2), e16–e25. DOI: 10.1016/j.jinf.2020.04.021
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