STUDYING THE GENETIC DIVERSITY OF SOME SOLANUM MELONGENA L. VARIETIES USING MOLECULAR TECHNOLOGY
DOI:
https://doi.org/10.54174/tg1as074Keywords:
Key Word: Eggplant , RAPD.Abstract
Using polymerase chain reaction (PCR)- based random amplified polymorphic DNA (RAPD) markers, the study conducted a genetic fingerprinting analysis of ten Eggplant Varieties Solanum melongena L. and measured the percentage of genetic distance. In the biotechnology laboratories of the Seed Inspection and Certification Department, during the fall season of 2024, using 5 primers from RAPD. Polymorphisms were of 74.75% detected among all genotypes, while the percentage of efficiency of each primer was 25.53% for the primer OPC-05, while the maximum discriminatory ability was 25.53% and 31.42% with primer OPC-05. The results showed that the highest percentage of similarity corresponds to the lowest genetic dimension (0.679) between Varieties S6 and S3, then the dimension (0.666) between Varieties S10 and S3. It was noted that the ten Varieties were distributed into two main groups A) and (B, as cluster A split into two subgroups (A1 and A2), and branch A1 included two secondary groups, the first of which included two genotypes: (S7, and S4) and the second was( S1 and S9), While group A2 included both types (S5 and S2). As for cluster (B), it was divided into two clusters, as the first cluster included both strains S3, S6, and S10, while the variety (S8) remained isolated from the other Varieties.
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