Detection of pathotype of Marek's Disease in layer chicken in Iraq
DOI:
https://doi.org/10.54174/utjagr.v12i2.284Abstract
Marek's Disease is a viral disease that affects poultry, particularly chickens. It is caused by the Marek's Disease Virus (MDV), which belongs to the herpesvirus family. The disease is characterized by the formation of tumors in various organs, including the nerves, skin, and internal organs such as the liver, spleen, and kidneys. A layer poultry farm of one day old, vaccinated with Marek's disease virus vaccine. The flock was infected with a virulent Marek's disease virus with a mortality rate of up to 60%. Histopathological examination of affected tissues is an important tool for the diagnosis of the disease. The histopathological finding of tissue sections from chickens with MDV shows extensive proliferation of lymphocytes of various sizes. The confirmation was done by PCR and standardized targeting a 132 bp tandem repeat region specific for serotype-1 MD viruses Meq gene was carried out by PCR and nucleotide sequencing, The sequence analysis of Meq gene of different clinical cases from Iraq homology with very virulent strain and Phylogenetic analysis of oncogenes was carried out with other available sequences in the GenBank we conclude that MDV strains obtained in the present outbreaks in Iraq could be designated as very virulent pathotypes based on nucleotide and phylogenetic analysis of the viruses.
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Othman I, Aklilu E. Marek’s disease herpesvirus serotype 1 in broiler breeder and layer chickens in Malaysia. Veterinary world. 2019; 12(3): 472 DOI: 14202/vetworld.2019.472-476
Eman Abdulali Al-Sereh, E.A.; Okash, A.N. and Ibrahim, M.A. (2020). The effect of foliar spray with pro.sol fertilizer and licorice extract on some vegetative growth indicators for young pomegranate (Punica granatum L.) seedlings cv. 'Salemi'. Int. J. Agricult. Stat. Sci., 16 (2): 739-744.
Shchebentovska O, М., Kostyniuk A. K, Veremchuk Y. Y., Khomenko Z. V., Fedyk, Y. Y., Revunets, A. S., Zaika, S. S. Pathomorphological changes in laying hens’ organs in case of infection by a field strain of the Marek’s disease virus. Ukrainian Journal of Ecology. 2021; 11(2): 122-129. DOI 10.15421/2021_88.
3. Kumar S, Kunec D, Buza J J, Chiang H I, Zhou H, Subramaniam S, Burgess S C. Nuclear Factor kappa B is central to Marek’s Disease herpesvirus induced neoplastic transformation of CD30 expressing lymphocytes in-vivo. BMC systems biology. 2012; 6(1): 1-19. DOI: http://www.biomedcentral.com/1752-0509/6/123
Burgess S C, Young J R , Baaten B J , Hunt L, Ross L N J , Parcells M S, Davison T F. Marek's disease is a natural model for lymphomas overexpressing Hodgkin's disease antigen (CD30). Proceedings of the National Academy of Sciences. 2004; 101(38), 13879-13884. DOI :10.1073/pnas.0305789101
Stamilla A, Messina A, Condorelli L, Licitra F, Antoci F, Lanza M , Puleio R. Morphological and immunohistochemical examination of lymphoproliferative lesions caused by Marek’s disease virus in breeder chickens. Animals. 2020; 10(8): 1280. DOI: 10.3390/ani10081280.
Abayli H, Karabulut B, Ozbek R, Ongor H , Timurkaan N, Tonbak S. Detection and molecular characterization of a highly oncogenic Marek’s disease virus from vaccinated hens in Turkey. Acta Virol. 2021; 65(2): 212-220. DOI:10.4149/av_2021_212.
Dunn J. R , Black Pyrkosz A, Steep A,Cheng H. H. Identification of Marek’s disease virus genes associated with virulence of US strains. Journal of General Virology. 2019; 100(7): 1132-1139. DOI: 10.1099/jgv.0.001288.
Molouki A, Ghalyanchilangeroudi A, Abdoshah, M Shoushtari, A Abtin, A Eshtartabadi, F, Nair, V. Report of a new meq gene size: The first study on genetic characterisation of Marek’s disease viruses circulating in Iranian commercial layer and backyard chicken. British Poultry Science. 2022; 63(2):142-149. DOI:10.1080/00071668.2021.1963677
Bertzbach L. D , Conradie A. M., You, Y., Kaufer B. B. Latest insights into Marek’s disease virus pathogenesis and tumorigenesis. Cancers. 2020; 12(3), 647. DOI :org/10.3390/cancers12030647
Carvallo F. R , French R A., Gilbert-Marcheterre K., Risatti G., Dunn, J. R , Forster F, Smyth, J. A. Mortality of one-week-old chickens during naturally occurring Marek’s disease virus infection. Veterinary pathology. 2011; 48(5): 993-998. DOI:10.1177/0300985810395727
Prathibha Y, Sreedevi B, Kumar N V, Srilatha, C. H. Molecular characterization and phylogenetic analysis of oncogenes from virulent serotype-1 Marek’s disease virus in India. Acta Virol. 2018; 62(3), 277-286.DOI: 10.4149/av_2018_221
AlZuhariy M. T. Isolation and identification of the newcastle disease virus from field outbreaks in broiler and layer flocks in Iraq.The Iraqi J. Vet. Med. 2017;41(1): 23–27.
Ozan E, Muftuoglu I, Sahindokuyucu H. N, Kurucay, S. Inal, Kuruca NA. E, Elhag E. Karaca, Tamer C, Gumusova S, Albayrak H, Barry G, Gulbahar MY, Yazici Z. Marek’s disease virus in vaccinated poultry flocks in Turkey: its first isolation with molecular characterization. Arch. Virol. 2021;166(2): 559-569. DOI: 10.1007/s00705-020-04943-6.
Liu L , Qu Y, Wang T, Wang G, Wang F, Liu S. Skin involvement in lymphomas caused by Marek’s disease virus infection in Silkie chickens. Journal of Veterinary Diagnostic Investigation. 2021; 26(2), 302-307. DOI: 10.1177/1040638714522462
Boodhoo, Gurung N, Sharif A , Behboudi S. Marek’s disease in chickens: a review with focus on immunology. Veterinary research. 2016;47(1), 1-19. DOI: 10.1186/s13567-016-0404-3
David E. Swayne, Martine Boulianne, Catherine M. Logue, Larry R. McDougald, Venugopal Nair, David L. Suarez, Sjaak de Wit, Tom Grimes, Deirdre Johnson, Michelle Kromm, Teguh Yodiantara Prajitno, Ian Rubinoff and Guillermo Zavala (Eds.) Diseases of poultry 14th edition. 2020. pp1451.DOI:10.1080/03079457.2020.1794237.
Abdul-Aziz , Oscar J F. Lymphoid System in: Avian histopathology.The American Association of Avian Pathologists;2016. 2-14p.
Shivaprasad H L, Barnes H J. Integumentary System in: Avian histopathology.The American Association of Avian Pathologists; 2016.67-637p
Krol K J, Samorek-Salamonowicz E, Kozdrun W , Wozniakowski G. Duplex PCR assay for detection and differentiation of pathogenic and vaccine strains of MDV serotype 1. Bull. Vet. Inst. Pulawy. 2007;51(3): 331-335. (aviable here).
Wajid S J, Katz M E, Renz K G,Walkden-Brown S. W. Prevalence of marek's disease virus in different chicken populations in Iraq and indicative virulence based on sequence variation in the EcoRI-Q (Meq) gene. Avian Dis. 2013; 57(2s1): 562-568 DOI:10.1637/10342-083112.
Ahmed A I,Odisho S. M. Isolation identification and pathotyping of newcastle disease viruses form naturally infected chickens in Iraqi Kurdistan region. Iraqi J. Agric. Sci. 2018; 49(1): 132-141.
Najem H A. Clinical, pathological and molecular study of marek’s disease in the Gallus gllus domesticus and brahma chickens of Basra province, Iraq. Egypt. J. Vet. Sci. 2021; 52(1): 63-71 10.21608/ejvs.2020.39336.1187.
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