The Impact of Two Types of Mycoplasma gallisepticum Vaccines on Broiler Chicken Respiratory System.
DOI:
https://doi.org/10.54174/utjagr.v12i2.273Keywords:
Mycoplasma ,poultry ,trachea, air sacs ,humoral immunity ,cellular immunity.Abstract
This study aimed to evaluate the effect of vaccinating 1-day-old broiler
chicks with two commercial vaccines such as live chronic respiratory
disease F strain (CRDF) and killed Mycoplasma gallisepticum (MG)
vaccines on the respiratory system. For this purpose, one-day-old broiler
chicks (n=120) were randomly divided into four groups; (n=30 birds
each). All groups were treated as followed: G1(CRDF): chicks were
vaccinated with live CRDF vaccine (eye drop one drop 0.03ml/dose/10 5 ),
G2 (killed MG): chicks were vaccinated with killed MG vaccine injection
with 0.3ml s.c /3 x10 10 C.F.U/ml), G3: chicks were combined vaccinated
with killed MG and CRDF live vaccines G4: served as control
unvaccinated group. The vaccination was performed at one day old in G1
(CRDF), G2(killed MG), and G3 (killed MG and live CRDF). The
histopathological changes of the trachea and air sacs were examined and
evaluated at 35 days old. The results of histopathological examination of
air sacs in the G1(CRDF) revealed various mild epithelial hyperplasia
with papillary-like growth, subepithelial lymphocytic infiltration, in G2
(killed MG), showed hyperplastic epithelial surfaces with moderate
lymphocytic infiltration mainly around congestion capillaries. While G3
(killed MG and CRDF) showed focal epithelial hyperplasia (villus-like),
and perivascular lymphocytic aggregation in laminar C.T. Whereas G4
(control) showed normal structures without any histopathological lesions.
In the trachea the histopathological changes in G1(CRDF) showed focal
lymphocytic aggregation with a follicle-like appearance, another section
showed focal epithelial and cilia loss with goblet cell hypertrophy of the
adjacent gland. G2 (killed MG) showed partial loss of thin mucosa with
mild and desquamation lymphocytic infiltration in lamina propria,
G3(killed MG and CRDF) showed diffuse mononuclear cells (MNCs)
infiltration in tracheal mucous and submucosa with evidence of cystic
dilution of adjacent gland together G4 control tracheal tissue showed
normal structure without any lesions. Finally, it was determined that
vaccination with killed MG and live CRDF vaccines caused a more
obvious immune reaction (lymphoproliferative infiltration) in the
respiratory system, including the trachea and air sac than the vaccination
with combined killed and live vaccines, while the live Mycoplasma
vaccines individually caused minor damage, particularly in the trachea.
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