First report of Fusarium subglutinans (Wollenw. & Reinking) (1983) as a causative agent of leaf spot disease on broad bean Vicia faba L. in Iraq

Authors

DOI:

https://doi.org/10.54174/utjagr.v12i2.259

Keywords:

broad bean, Fusarium subglutinans ITS, molecular diagnosis phenotypic

Abstract

  Broad bean (Vicia faba L.) is one of the most common vegetable crops in Iraq; cultivated in many areas. Broad bean production has been affected significantly by several diseases caused by either bacteria or fungi; among these pathogens Fusarium have a direct impact on faba beans production. The current study was conducted at the laboratories of the College of Agriculture - University of Basrah to identify the fungal pathogens of broad bean leaf spot disease. Isolation of the fungal pathogen was performed on PDA and PCA media; the morphological and microscopic identification revealed the identity of Fusarium subglutinans, followed a molecular diagnosis by ITS universal primers (ITS1 and ITS4) with  a similarity percentage of 99%; the gene sequence was deposited at NCBI as LC769974.

 Experimental tests confirmed the pathogen's capability to infect the vegetative system of the broad bean plants. This is the first recorded of F. subglutinans being identified as the cause of leaf spotting on broad bean plants, making it a notable discovery in both Iraq and globally.

Downloads

Download data is not yet available.

References

Ahmed, A. N., & Abass, M. H. (2022). Disease Note: First Report of Cladosporium ramotenellum Schub., Zalar, Crous & Braun, 2007 (Fungi: Dothideomycetes) as a Potential Contaminant of Date Palm Tissue Culture. Basrah Journal of Agricultural Sciences, 35(2), 373-375

Arya, R. K.; Kumar, R.; Hooda, J. S.; Sutaliya, J.; Dahiya, M.;Vandana,G. S.; Raiger,H. L. ;Yadav, S. K.; Gill, R. K.; Mehto, J. L.; Tiwari, J. K.; Yadav, C. B.; Deen, M. K.; Punia,R. ; Raj,K.; Lal, R.; Kumar, P.; Tripathi, R. N.; Singh,G.; Singh,S. P.( 2022) Pre-breeding evaluation of germplasm for hybridization and screening of resulting transgressive elite genotypes faba bean for yield and its attributes for semi-arid regions of India. Ekin journal of crop breeding and genetics, 8(1) :17- 26

Borras, O., Santos, R., Matos, A. D., Cabral, R. S., & Arzola, M. (2001). A first attempt to use a Fusarium subglutinans culture filtrate for the selection of pineapple cultivars resistant to fusariose disease. Plant Breeding, 120(5), 435-438

Crépon, K., Marget, P., Peyronnet, C., Carrouee, B., Arese, P., Duc, G. (2010). Nutritional value of faba bean (Vicia faba l.) seeds for feed and food. Field Crops Research, 115 (3), 329–339.

Qahtan, A.A.; Al-Atar, A.; Abdel-Salam, E.M.; El-Sheikh, M.A.; Gaafar, A.-R.Z.; Faisal, M.(2021) Genetic diversity and structure analysis of a worldwide collection of faba bean (Vicia faba) genotypes using ISSR markers. International Journal of Agriculture And Biology, 25, 683–691

Han, K. S., Park, J. H., Back, C. G., & Park, M. J. (2015). First report of Fusarium subglutinans causing leaf spot disease on Cymbidium orchids in Korea. Mycobiology, 43(3), 343-346

Harish, J., Jambhulkar, P. P., Bajpai, R., Arya, M., Babele, P. K., Chaturvedi, S. K., ... & Lakshman, D. K. (2023). Morphological characterization, pathogenicity screening, and molecular identification of Fusarium spp. isolates causing post-flowering stalk rot in maize. Frontiers in Microbiology, 14, 1121781

Ichikawa K., Aoki T. 2000. New leaf spot disease of Cymbidium species caused by Fusarium subglutinans and Fusarium proliferatum. Journal of General Plant Pathology 66 (3): 213–218

Martineau-Côté, D., Achouri, A., Karboune, S., & L’Hocine, L. (2022). Faba bean: an untapped source of quality plant proteins and bioactives. Nutrients, 14(8), 1541

Shin, J. H., Han, J. H., Lee, J. K., & Kim, K. S. (2014). Characterization of the maize stalk rot pathogens Fusarium subglutinans and F. temperatum and the effect of fungicides on their mycelial growth and colony formation. The plant pathology journal, 30(4), 397

Hussein Abbas Jebur. (2023). A review: Machine relationship with the tractor and its effect on the productivity and compaction of agricultural soil. University of Thi-Qar Journal of Agricultural Research, 12(1), 22–43. https://doi.org/10.54174/utjagr.v12i1.237

Yaser, H. S., & Abass, M. H. (2022). Morphological and molecular identification study of rose and lantana fungal leaf spot pathogens. Basrah Journal of Science, 40(2), 342-356

Muhsin N. Hoshan, & Muhammad M. Yassin. (2023). The interaction effect of leaching requirements, salinity of irrigation water, levels of added sulfur and type of organic matter on soil salinity cultivated with maize plants (Zea mays L.) . University of Thi-Qar Journal of Agricultural Research, 12(1), 1–21. https://doi.org/10.54174/utjagr.v12i1.228

Downloads

Published

2023-10-01

How to Cite

Baidaa G. Ofi, Mohammed H. Abass, & Yehya A. Salih. (2023). First report of Fusarium subglutinans (Wollenw. & Reinking) (1983) as a causative agent of leaf spot disease on broad bean Vicia faba L. in Iraq. University of Thi-Qar Journal of Agricultural Research, 12(2), 41–45. https://doi.org/10.54174/utjagr.v12i2.259

Issue

Section

Articles