Effect of glutathione and ascorbic acid on some physical characteristics of seedlings of grape plant Halawani cultivar Vitis vinifera L.

  • Mahmood S. Abdel-Wahed
  • Lina A. Hameed

Abstract

This experiment was conducted in the saran-covered plastic house of the College of Agriculture and Marshes, Thi-Qar University, on 20.02.2022, to study the effect of spraying with glutathione and ascorbic acid on the physical and chemical properties of the grape plant Vitis vinifera L at one year age. The effect of glutathione compound at four concentrations (0, 75, 150, 225) mg.l-1 and ascorbic acid at four concentrations also (0, 50, 100, 150) mg.l-1, and the interaction between them was studied and implemented as a factorial experiment with two-factors (4×4×3) by following the randomized complete block design (RCBD) with three replications (48 seedlings per replicate). The averages were tested using the Revised least significant difference R.L.S.D at the probability level of 5%. The most important results obtained indicate that the effect of spraying with glutathione significantly on the studied vegetative characteristics, where the spraying treatment (G3) with a concentration of (225 mg l-1) was significantly superior to the control treatment (G0) in(Stem diameter, number of branches, dry weight, wet weight), which gave the highest averages, respectively ((24,901 mm, 7.942 branches. plant-1, 1.596 g, 0.403 g, 20.903), respectively. As for ascorbic acid, its effect was significant. In the studied vegetative traits, the spraying treatment (3C) with a concentration of (150 mg.L-1) was superior in (plant height (cm), number of leaves (leaf. plant-1), leaf area (cm2), which gave the highest averages, respectively ( 25.110 mm, 25.110 mm, 7.509 branches. plant-1,1.649 g, 0.392, 20.767).As for the two interactions (glutathione + ascorbic acid) at a concentration of (225 mg.l-1 + 150 mg.l-1), the treatments showed clear statistical differences, as the treatments excelled in the vegetative and chemical characteristics (stem diameter, number of branches, wet weight, weight). Dry, chlorophyll content of leaves) was a significant effect on the control treatment

References

1. Abdul-Qader, SM .2006. Effect of training systems, canopy management and sampling dates on the yield and quality of grapevines CV "Taifi" Vitis vinifera L. under non-irrigated conditions M. Sc.Thesis.college of Agriculture, University of Duhok.
2. Qassem, Hassan Ali and Rashid Sultan Al-Obeid Al-Saeedi, Ibrahim Hassan Mohammed (2014). Grapes Classification, Dar Al-Wadah Publishing and Ishtar for Cultural Investments, The Hashemite Kingdom of Jordan, Amman.
3. Amini, A.A. ; Fatma, A. E. ; Gharib, M. ; El-Awadi and Rashad, M. (2011). Physiological response of onion plants to foliar application of putrescine and glutamine, Scientia Horticulturae, (129):353360.
4. 
5. Al-Saeedi, Ibrahim Hassan Muhammad (2014). Grapes Classification, Dar Al-Wadah Publishing and Ishtar for Cultural Investments, The Hashemite Kingdom of Jordan, Amman.
6. Bianucci, E., Tordable, M. D. C., Fabra, A., and Castro, S. (2008). Importance of glutathione in the nodulation process of peanut (Arachis hypogaea). Physiologia Plantarum, 134(2): 342-347.
7. Blokhina, O.; E. Virolainen and K.V. Fagerstedt (2003). Antioxidants, oxidative damage and oxygen deprivation stress: A review. Annals of Botany, 91: 179-194.
8. El-Kobisy, D.S.; K.A. Kady, R.A. Medani and R.A. Agamy (2005). Response of Pea plant (Pisum sativum L.) to treatment with ascorbic acid. Egypt J. Appl. Sci., 20: 36-50.
9. EL-Naggar, A.H. and E., Sewedan, 2009. Effect of light intensity and amino acid tryptophan on the growth and flowering of Amaryllis (Hippeastrum vittatum, HERB.) plants, J. Agric. and Euv. Sci. Alex. Univ., Egypt. 8(1).
10. Galal, A.A., S.H., Gad El-Hak, Y.Y., Abdel-Ati and Y.M.M., Moustafa, 2000. Response of new tomato hybrids to some antioxidants and early blight. The 2 Scientific Conference 2nd of Agricultural Sciences, Assult, Egypt, pp: 673-686
11. Noctor, G., Mhamdi, A., Chaouch, S., Han, Y. l., Neukermans, J., Marquez-Garcia, B. E. L. E. N. & Foyer, C. H. (2012). Glutathione in plants: an integrated overview. Plant, cell & environment, 35(2), 454-484.
12. Smith , T.A. , 1985. Polyamines . Annu . Rev. Plant Physiol . , 36 : 117-143 .
13. Hussein ,M.M. ; Okasha , E.M. and Mehanna , H.M. (2014).Response of Cotton Plants to Glutathione Rates under Saline Conditions .Middle East journal of Appl. sciences 4(1):47-53.
14. Gharib, F. A. and Hegazi, A. Z. (2010). Salicylic acid ameliorates germination, seedling growth, phytohormones and activity in bean (Phaseolus vulgaris L.) under cold stress. J. Amer. Sci., 6(10):675-683.
Published
2022-11-03
Section
Articles