Study of the Level of Gene Expression of the GLUT2 Glucose Vector in the Small Intestine of the Meat Broiler and its Relationship to Productive Traits
DOI:
https://doi.org/10.54174/utjagr.v11i2.182Abstract
The study was conducted in the field of animal production of the Agricultural Research Station / College of Agriculture and the Marshes / Dhi Qar University for the period from 10/11/2021 to 25/ 2/ 2022. Laboratory tests of the samples were carried out in the Molecular Genetics Laboratory of the College of Dentistry / Dhi Qar University, for the purpose of knowing the gene expression of the SLC2A2 gene encoding the glucose transporter GLUT2 and the abundance of its mRNA transcripts in different regions of the small intestine of broilers when fed at different levels of energy and to study the relationship of that abundance to some productive traits.
In this study, 135 non-naturalized of meat broiler (breed 308 Ross) were used. The broiler were randomly distributed to three experimental treatments, with three replicates for each group (15 chicks/repeat), used the adlibitum free feeding system for the duration of the experiment, which lasted (35) days. The study included three levels of energy, where the first treatment was a control diet, which represented 3065 and 3133 kilocalories/kg for the primer and final diet, respectively, as for the second treatment, it contained a high level of energy 3200 kilocalories/kg and 3297 kilocalories/kg for the primer and final diets, respectively, the third treatment contained a low level of energy 2769 kilocalories/kg and 2802 kilocalories/kg for primer and growth diets, respectively. The results of the study showed the following:-
- The possibility of amplifying the studied gene and knowing its abundance and according to the primers used.
- The highest level of mRNA transcription of the GLUT2 gene in the small intestine of males was in the jejunum region (9.41) at a representative energy level of 3297 kilocalories/kg, as for the duodenum, the expression of GLUT2 gene was lower than in the jejunum (0.535), the GLUT2 gene expression in males was slightly more than in females in the ileum region (0.578) at a representative energy level of 3297 kcal/kg.
- Gene expression was positively and significantly associated with live weight, weight gain and feed consumption rates, but it was inversely related to the efficiency of food conversion in the jejunum and duodenal regions.
- The abundance of the glucose transporter GLUT2 gene increased with the increase in the energy level in the diet.
- Males gave a higher percentage of mRNA copies in all target organs compared to females.
- The activity of the gene responsible for the transfer of glucose can be modified through feeding and increasing energy levels and thus its reflection on the performance of birds.
- As for the productive traits, the differences were significant in the average live body weight and weight gain, as well as feed consumption compared with the control treatment, while the feed conversion efficiency did not show any significant differences.
Downloads
References
Abbas, M. A. F., & Ibrahim, M. A. (1996). The role of ethylene in the regulation of fruit ripening in the Hillawi date palm (Phoenix dactyliferaL). Journal of the Science of Food and Agriculture, 72(3), 306-308.
Ibrahim, M. A., Al-Taha, H., & Seheem, A. A. (2013). Effect of cytokinin type and concentration, and source of explant on shoot multiplication of pineapple plant (Ananas comosus' Queen') in vitro/Ucinek vrst in koncentracij citokininov ter vira stebelnih izseckov na in vitro razmnozevanje ananasa (Ananas comosus' Queen'). Acta agriculturae Slovenica, 101(1), 15.
Ibrahim, M. A. (2012). In vitro plant regeneration of local pummelo (Citrus grandis (L.) Osbeck.) via direct and indirect organogenesis. Genetics and Plant Physiology, 2(3-4), 187-191.
Ibrahim, M. A., & Daraj, I. A. (2015). Micropropagation of dahlia plants Dahlia variabilis Wild (Desf.). Effect of explant and plant growth regulators on shoot regeneration and growth. Advances in Agriculture & Botanics, 7(1), 1-6.
Ibrahim, M. A., Waheed, A. M., & Al-Taha, H. A. (2013). Plantlet regeneration from root segments of date palm tree (Phoenix dactylifera L. cv. Barhee) producing by in vitro culture. Advances in Agriculture & Botanics, 5(1), 45-50.
Abd Elhamid, M. I., Makboul, H. E., Sedik, M. Z., Ismail, I. M., & Ibrahim, M. A. (2010). Cloning, expression and antifungal activity of an endochitinase gene derived from barley (Hordeum vulgare). Research Journal of Agriculture and Biological Sciences, 6(3), 356-363.
Sharaf, A. N., Soliman, M. H., Ibrahim, M. A., Moustafa, S. A., & Hadi, A. (2007). Molecular identification and cloning of organophosphate degradation gene in some bacterial isolates. Arab Journal of Biotechnology, 10(2), 259-274.
Raza, T., Ibrahim, M., & Amjad, M. (1999). Seasonal changes in mineral nutrient and seed oil in Kinnow fruit (Citrus reticulata Blanco). Int. J. Agric. Biol, 4, 339-341.
Al-Sereh, E. A., Okash, A. N., & Ibrahim, M. A. (2020). THE EFFECT OF FOLIAR SPRAY WITH PRO. SOL FERTILIZER AND LICORICE EXTRACT ON SOME VEGETATIVE GROWTH INDICATORS FOR YOUNG POMEGRANATE (PUNICA GRANATUM L.) SEEDLINGS CV.'SALEMI'. Int. J. Agricult. Stat. Sci, 16(2), 739-74.
Abbas, M. F., & Ibrahim, M. A. (1998). Effect of pollen parent on the physiology of ripening of date palm fruit (Phoenix dactylifera L). Basrah J. Agric. Sci, 11(2), 31-37.
Ibrahim, M. A., Al-Taha, H. A., Saaid, Z. A., & Assist, R. PROPAGATION OF STRAWBERRY VIA IN VITRO ADVENTITIOUS SHOOT FORMATION.
Ibrahim, M. A., Metry, E. A., Moustafa, S. A., Tawfik, A. E., & Nasr El-Din, T. M. (2004). Morphological and molecular diagnosis of commercial potato cultivar infected with ralostonia solanacearum. Arab J Biotechnol, 8, 211-222.
Alnajjar, M. A., Alpresem, W. F., & Ibrahim, M. A. (2020). Effect of amino acid proline treatment on anatomical characteristics of leaves and roots of date palm seedlings Phoenix dactylifera L. developed under saline stress conditions. Plant Archives, 20(1), 755-760.
Ibrahim, M. A., Jasim, A. M., & Abbas, M. F. (2012). In vitro plant regeneration of Indian jujube (Ziziphus mauritiana Lamk.) cv. Zaytoni via indirect organogenesis. Acta Agric. Slov, 99(1), 65-67.
Ibrahim, M. A., Jasim, A. M., & Abbas, M. F. (2012). In vitro plant regeneration of Indian jujube (Ziziphus mauritiana Lamk.) cv. Zaytoni via indirect organogenesis. Acta Agric. Slov, 99(1), 65-67.
Ibrahim, M. (2022). Role of Endogenous and Exogenous Hormones in Bioactive Compounds Production in Medicinal Plants via In Vitro Culture Technique. Plant Hormones-Recent Advances, New Perspectives and Applications.
Al-Hamad, A. D. S., Alnajjar, M. A. H., & Ibrahim, M. A. (2021). The effect of alternating salinity of irrigation water and cultivation level on some soil properties and growth of date palm (Phoenix dactylifera L.) offshoots of Hillawi cultivar in clay soils. Int. J. Agricult. Stat. Sci. Vol, 17(1), 63-72.
Ibrahim, M. A., & Al-Sereh, E. A. (2019). Effect of foliar spray with potassium humate and green tea extract on some of the vegetative characteristics of guava (Psidium guajava L. cv. Local) seedlings. Plant Archives, 19(1), 404-408.
Ibrahim, M. A., Jerry, A. N., & Khalil, A. I. (2018). Effect of salt stress on some chemicals characteristics of callus for three cultivars of potato plant (Solanum tuberosum L.). Advances in Agriculture & Botanics, 10(2), 87-96.
Ibrahim, M. A., Jerry, A. N., & Khalil, A. I. (2017). Effect of plant growth regulators and sodium chloride on indirect organogenesis of three cultivars of potato plant (Solanum tuberosum L.) via in vitro culture. Advances in Agriculture & Botanics, 9(3), 103-110.
Safana, H. S., Ibrahim, M. A., & Abd, A. M. (2022). Impact of chitosan and benzyl adenine on shoot multiplication of kumquat plant (Citrus japonica Thumb.) in vitro. Int. J. Agricult. Stat. Sci. Vol, 18(1), 359-365.
Ibrahim, M., Ali, A. H., & Hashem, M. S. (2021). In vitro effects of cyanobacteria (Oscillatoria tenuis) extracellular products on date palm (Phoenix dactylifera L. cv.‘Barhee’) propagation. DYSONA-Applied Science, 2(1), 1-7.
Ibrahim, M. A., & Yassin, M. M. (2020). Direct shoot regeneration by in vitro culture of the gerbera (Gerbera jamesonii Bolus) capitulum explants. Plant Cell Biotechnol Mol Biol, 21, 1-9.
Salih, A. M., Alaradi, H. J., Ibrahim, M. A., & Alhello, A. A. (2019). Effect of some methods of planting in guava (Psidium guajava L.) seed germination. Plant Archives (09725210), 19(2).
Ibrahim, M. A., & Hamzah, H. A. (2019). Effect of the harvest date, storage period and package on the storability of date palm (phoenix dactylifera L. Cv. Taberzal) fruits. Plant Archives, 19(2), 226-231.
Taain, D. A., Ibrahim, M. A., & Al-Sareh, E. A. (2011). A study on the effect of postharvest calcium chloride treatments on the storability of mango fruits (Mangifera indica L.). Dirasat, 5, 37.
Abbas, M. F., Ibrahim, M. A., & Jasim, A. M. (2014). Micropropagation of Indian jujube (Ziziphus mauritiana Lam. cv. Zaytoni) through shoot tip culture. Advances in Agriculture & Botanics, 6(1), 11-15.
Ibrahim, M. A., Al-Taha, H., & AL-ALI, Z. T. (2017). Effect of casein hydrolysate and paclobutrazol on embryogenesis of date palm (Phoenix dactylifera L.) cv. Nersy by in vitro culture. Journal of Garmian University, 4(ICBS Conference), 395-404.
Ibrahim, M. A., Al-Aradi, H. J., & Muhsen, K. A. (2017). Effect of some growth regulators on proliferation of myrtle (Myrtus communis L. cv. Local) plant by in vitro culture. Advances in Agriculture & Botanics, 9(2), 97-101.
Ibrahim, M., & Yassin, M. M. (2021). Adventitious shoot proliferation from callus of Gerbera jamesonii young leaf explant. DYSONA-Applied Science, 2(4), 47-52.
SAFANA, H. S., ABD, A. M., & IBRAHIM, M. A. (2021). Study of genetic variations of In vitro micropropagation stages of Kumquat (Citrus japonica Thunb.). PLANT CELL BIOTECHNOLOGY AND MOLECULAR BIOLOGY, 292-299.
AL-JABIR, H. S. S., ABDULLA, A. A., & IBRAHIM, M. A. A STUDY OF SOME BIOACTIVE COMPONENTS IN WILD MALVA (Malva parviflora L.) PLANT IN DIFFERENT LOCATIONS OF BASRAH, SOUTHERN IRAQ.
Safana, H. S., Ibrahim, M. A. H. I., & Abd, A. K. M. (2022). Effect of NAA and Chitosan in rooting branches resulting from stem nodes plantation of Kumquat (Citrus japonica) in vitro. Journal of Kerbala for Agricultural Sciences, 9(1), 7-18.
Salih, A. M., Ibrahim, M. A., Al-Aradi, H. J., & Abass, M. H. (2020). The response of different date palm (Phoenix dactylifera L.) cultivars to callus induction and development by in vitro culture under salt stress. POLISH POLAR RESEARCH, 41(11), 23-41.
Ibrahim, M. A., Mohsen, A. T., Abdel-Mohsen, M. A., & Mostafa, A. S. (2014). Response of some Citrus rootstock seedlings to stimulating growth applications. Egypt. J. Hort, 41(2), 347-363.
Al–Soudi, K. A. (1971). The Poultry Industry in Iraq. World's Poultry Science Journal, 27(4), 380-385.
Benyi, K., Tshilate, T. S., Netshipale, A. J., & Mahlako, K. T. (2015). Effects of genotype and sex on the growth performance and carcass characteristics of broiler chickens. Tropical Animal Health and Production, 47(7), 1225-1231.
Dekkers, J. C. (2004). Commercial application of marker-and gene-assisted selection in livestock: strategies and lessons. Journal of animal science, 82(suppl_13), E313-E328.
Emmerson, E. R., Li, H., Gilbert , D. A., Webb Jr, K. E., & Wong, E. A. (2008). Dietary protein quality and feed restriction influence abundance of nutrient transporter mRNA in the small intestine of broiler chicks. The Journal of nutrition, 138(2), 262-271.
Garriga, C., Barfull, A., & Planas, J. M. (2004). Kinetic characterization of apical D-fructose transport in chicken jejunum. The Journal of membrane biology, 197(1), 71-76.
Abdulkareem, Ali A., Ayid, Asaad Y., & Mohamed, Abdullah A. (2016) Study mRNA Abundance and the Gene Expression Gene Expression of Energy Transporter Genes in The Small Intestine of Broiler of Different Levels of Protein and Energy in Diets. Journal of agricultural research 159-174V. 1(5)
Kaminski, N. A., & Wong, E. A. (2018). Differential mRNA expression of nutrient transporters in male and female chickens. Poultry science, 97(1), 313-318.
NRC . National Research Council . 1994 . Nutrient Requirements of Poultry , 9th ed., National Acad . Press , Washington , D . C . : NAS , 155.Nutr.NutrientTransporter Gene Expression In The Small Intestine of
Rinttilä, T., & Apajalahti, J. (2013). Intestinal microbiota and metabolites—Implications for broiler chicken health and performance. Journal of Applied Poultry Research, 22(3), 647-658.
Wood, I. S., & Trayhurn, P. (2003). Glucose transporters GLUT and SGLT): expanded families of sugar transport proteins. British journal of nutrition, 89(1), 3-9.
Al-Zubaidi, Suhaib Saeed Alwan, 1986. Poultry Management, Mosul University Press
Al Fayadh, Hamdi Abdel Aziz Saad Abdel Hussein Naji and Nadia Nayef Abdel Hajo, 2011. Poultry Products Technology. University of Baghdad, College of Agriculture, Higher Education Press, University of Baghdad, Part One, Second Edition.
Khasha Mahmoud Al-Rawi Abdulaziz Muhammad Khalaf Allah "Design and Analysis of Agricultural Experiments", Dar Al-Kutb for printing and publishing. / Mosul University (1980).
Saeed A. Mseer, Mahmoud S. Al-Ibrahimi and Falah H. Al-Miahy (2020). The effect of spraying with Nano fertilizers NPK and Gibberellin GA3 on the physical, chemical and physiological characteristics on the vegetative growth and fruit yield of Fig trees, black Diyala cultivar Ficus carica L. International Journal of Agricultural and Statistical Sciences. DocID: https:// connectjournals.com/03899.2020.16.1793
Maytham M. Kazem, Mahmoud S. Abdul Wahid and Ihssan J. Dhabib (2020). The effect of spraying with nutrient solution and organic fertilization on the vegetative and chemical characteristics of seedlings of domestic sour lemon (Citrus Limon L.). International Journal of Agricultural and Statistical Sciences. DocID: https://connectjournals.com/03899.2020.16.1787
Abdul-Wahid, M. S., Aqeel, H. A., & Rawaa, H. H. (2012). Effect of spray by Ascorbic and salicylic acid on some physiochemical properties of the local sour orange seedling citrus aurantium L. Thi-Qar University Journal for Agricultural Researches, 2(2222-5005).
Abdel-Wahed, M. S., & Mohsen, M. G. (2022). Effect of spraying with Ferti Mar and GROWX AMINO MIX on some chemical characteristics of Citrus aurantium seedlings Citrus aurantium L. University of Thi-Qar Journal of agricultural research, 11(2), 131-141.
Abdel-Wahed, M. S., & Hameed, L. A. (2022). Effect of glutathione and ascorbic acid on some physical characteristics of seedlings of grape plant Halawani cultivar Vitis vinifera L. University of Thi-Qar Journal of agricultural research, 11(2), 122-130.
ISO 690
Faiza K. Al-Rumaiydh, Falah H. Al-Miahy and Mahmud S. Ebd Alwahid (2020). The effect of Adding Nano NPK and Spraying by Salicylic Acid on some of the Major and Minor Metabolism Products of Pomegranate Seedlings, Cultivar Wonderful in Dhi-Qar Governorate. International Journal of Agricultural and Statistical Sciences. DocID: https://connectjournals.com/ 03899.2020.16.2051
Abdul-wahid, M. S. (2019). Effect of Spray by Chelated Iron in physical and Chemical Characteristics of Iraqi Banana Musa Paradisica L. Var Sapientum (L.) Oktze Cultivation South Iraq. University of Thi-Qar Journal of agricultural research, 8(1).
Abdul-Wahid, M. S., & Abdulla, N. A. (2012). Effect of Iron Injection on some chemical Properties and yield of Date palm (phoenix dactylifera L.) cv kudrawe. Thi-Qar University Journal for Agricultural Researches, 1(2222-5005).
Jwar, A. S., & Abdul-Wahid, M. S. (2018). Effect of different pollen grain source and chitosan on some physiological and enzymatic characteristics of data palm Phoenix dactylifera L. fruits cv. Alshwethey. Basrah Journal For Date Palm Research, 17(1-2).
Abdul-wahid, M. S., & Jawar, A. (2019). Response of Some chemical, physical and productivity propertiesto date palmPhoenix dactylifera L. cultivation sites. University of Thi-Qar Journal of agricultural research, 8(1).
Abdul-wahid, M. S. (2019). Effect of Spray by Chelated Iron in physical and Chemical Characteristics of Iraqi Banana Musa Paradisica L. Var Sapientum (L.) Oktze Cultivation South Iraq. University of Thi-Qar Journal of agricultural research, 8(1).
-Jwar, A. S., & Neghamish, R. G. (2021). Effect of levels organic fertilizers and kinds of Bio-feritilizers in characterical of two classes of Okra plant (Abelmoschus esculentus L.) growning in the plastic house. University of Thi-Qar Journal of agricultural research, 10(1), 54-69.
ISO 690
-Jwar, A. S., Wahed, M. S. A., & Ghani, S. T. (2021). Study the effect of foliar spraying with Dionyfer and Azomin on the vegetative growth indicators of the leaves of Henna plant (Lawsonia inermis L). University of Thi-Qar Journal of agricultural research, 10(1), 70-78.
Connectjournals
Journals Directory India - Directory of Journals from India, Journals List
journals directory india - connectjournals.com a directory of journals from india also provides list of e-journals, e-publishing, journal subscription...
Downloads
Published
Issue
Section
License
Copyright (c) 2022 Anwar Hammood Obaid Al-Omar
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
1.png){kind=spacer}
