Study of the Level of Gene Expression of the GLUT2 Glucose Vector in the Small Intestine of the Meat Broiler and its Relationship to Productive Traits

  • Anwar Hammood Obaid Al-Omar


The study was conducted in the field of animal production of the Agricultural Research Station / College of Agriculture and the Marshes / Dhi Qar University for the period from 10/11/2021 to 25/ 2/ 2022. Laboratory tests of the samples were carried out in the Molecular Genetics Laboratory of the College of Dentistry / Dhi Qar University, for the purpose of knowing the gene expression of the SLC2A2 gene encoding the glucose transporter GLUT2 and the abundance of its mRNA transcripts in different regions of the small intestine of broilers when fed at different levels of energy and to study the relationship of that abundance to some productive traits.

In this study, 135 non-naturalized of meat broiler (breed 308 Ross) were used. The broiler were randomly distributed to three experimental treatments, with three replicates for each group (15 chicks/repeat), used the adlibitum free feeding system for the duration of the experiment, which lasted (35) days. The study included three levels of energy, where the first treatment was a control diet, which represented 3065 and 3133 kilocalories/kg for the primer and final diet, respectively, as for the second treatment, it contained a high level of energy 3200 kilocalories/kg and 3297 kilocalories/kg for the primer and final diets, respectively, the third treatment contained a low level of energy 2769 kilocalories/kg and 2802 kilocalories/kg for primer and growth diets, respectively. The results of the study showed the following:-

  • The possibility of amplifying the studied gene and knowing its abundance and according to the primers used.
  • The highest level of mRNA transcription of the GLUT2 gene in the small intestine of males was in the jejunum region (9.41) at a representative energy level of 3297 kilocalories/kg, as for the duodenum, the expression of GLUT2 gene was lower than in the jejunum (0.535), the GLUT2 gene expression in males was slightly more than in females in the ileum region (0.578) at a representative energy level of 3297 kcal/kg.
  • Gene expression was positively and significantly associated with live weight, weight gain and feed consumption rates, but it was inversely related to the efficiency of food conversion in the jejunum and duodenal regions.
  • The abundance of the glucose transporter GLUT2 gene increased with the increase in the energy level in the diet.
  • Males gave a higher percentage of mRNA copies in all target organs compared to females.
  • The activity of the gene responsible for the transfer of glucose can be modified through feeding and increasing energy levels and thus its reflection on the performance of birds.
  • As for the productive traits, the differences were significant in the average live body weight and weight gain, as well as feed consumption compared with the control treatment, while the feed conversion efficiency did not show any significant differences.


1. Al–Soudi, K. A. (1971). The Poultry Industry in Iraq. World's Poultry Science Journal, 27(4), 380-385.‏
2. Benyi, K., Tshilate, T. S., Netshipale, A. J., & Mahlako, K. T. (2015). Effects of genotype and sex on the growth performance and carcass characteristics of broiler chickens. Tropical Animal Health and Production, 47(7), 1225-1231.‏
3. Dekkers, J. C. (2004). Commercial application of marker-and gene-assisted selection in livestock: strategies and lessons. Journal of animal science, 82(suppl_13), E313-E328.‏
4. Emmerson, E. R., Li, H., Gilbert , D. A., Webb Jr, K. E., & Wong, E. A. (2008). Dietary protein quality and feed restriction influence abundance of nutrient transporter mRNA in the small intestine of broiler chicks. The Journal of nutrition, 138(2), 262-271.‏
5. Garriga, C., Barfull, A., & Planas, J. M. (2004). Kinetic characterization of apical D-fructose transport in chicken jejunum. The Journal of membrane biology, 197(1), 71-76.‏
6. Abdulkareem, Ali A., Ayid, Asaad Y., & Mohamed, Abdullah A. (2016) Study mRNA Abundance and the Gene Expression Gene Expression of Energy Transporter Genes in The Small Intestine of Broiler of Different Levels of Protein and Energy in Diets. Journal of agricultural research 159-174V. 1(5)
7. Kaminski, N. A., & Wong, E. A. (2018). Differential mRNA expression of nutrient transporters in male and female chickens. Poultry science, 97(1), 313-318.
8. NRC . National Research Council . 1994 . Nutrient Requirements of Poultry , 9th ed., National Acad . Press , Washington , D . C . : NAS , 155.Nutr.NutrientTransporter Gene Expression In The Small Intestine of
9. Rinttilä, T., & Apajalahti, J. (2013). Intestinal microbiota and metabolites—Implications for broiler chicken health and performance. Journal of Applied Poultry Research, 22(3), 647-658.‏
10. Wood, I. S., & Trayhurn, P. (2003). Glucose transporters GLUT and SGLT): expanded families of sugar transport proteins. British journal of nutrition, 89(1), 3-9.‏
11. Al-Zubaidi, Suhaib Saeed Alwan, 1986. Poultry Management, Mosul University Press
12. Al Fayadh, Hamdi Abdel Aziz Saad Abdel Hussein Naji and Nadia Nayef Abdel Hajo, 2011. Poultry Products Technology. University of Baghdad, College of Agriculture, Higher Education Press, University of Baghdad, Part One, Second Edition.
13. Khasha Mahmoud Al-Rawi Abdulaziz Muhammad Khalaf Allah "Design and Analysis of Agricultural Experiments", Dar Al-Kutb for printing and publishing. / Mosul University (1980).