Protective Role of Polyphenolic Compounds of Cyperus Rotundus and Taurine on Troponin –I and Lipid Profile of Female Rats treated with Isoproterenol – Induced Myocardial Infraction.

  • Alyaa Majid Department of Chemistry, College of Science, University of Thi-Qar, Iraq.
  • Mohammed A . Auda Department of Chemistry, College of Science, University of Thi-Qar, Iraq.
  • Khalid G . Al-Fartosi Department of Biology, College of Science, University of Thi-Qar, Iraq.
Keywords: Taurine, isoproterenol (ISO), therogenic index, Myocardia linfarction

Abstract

The present study was designed to investigate the effects of polyphenolic compounds extracted from tubers of  cyperus rotundus and taurine on isoproterenol induced myocardial infraction in female rats. Sixty of female rats (Rattus norvegicus) were used in the present study. Animals were divided into 10 groups ( 6 for each group). MI was induced in rats with ISO (85 mg/kg) twice at an interval of 24 hrs. ISO produced significant alterations in the troponin-I, cholesterol (TC), triglycerides (TG), HDL, vLDL, LDL and atherogenic index. The effect of polyphenolic compounds from tuberus of  cyperus rotundus oral treatment for 21 days at two doses (15 mg and 30 mg/kg, body weight) and taurine at two doses ( 100 mg and 200 mg/kg, body weight) was evaluated against ISO- induced cardiac necrosis. The results showed a significant increase in the level of serum troponin-I, cholesterol(TC), triglycerides(TG), vLDL, LDL and atherogenic index in ISO- treated animals as compared with the control animals. While, the results showed asignificant decrease in the level of serum HDL in ISO- treated animals as compared with the control animals. Polyphenolic compounds from tuberus of  cyperus rotundus and taurine showed a significant cardioprotective activity by lowering the levels of serum troponin-I,  cholesterol(TC), triglycerides(TG), vLDL, LDL and atherogenic index and elevate the levels of serum HDL. This study concluded that the polyphenolic compounds from tuberus of cyperus rotundus and taurine possess cardioprotective activity against Isoproterenol induced myocardial infraction in rats.

References

Abdel-Reheim, E . S. (2016). Cardioprotective efficacy of taurine on lipid- metabolism of Isoproterenol induced myocardial infraction. Nternational Journal of Pharmacy and Pharmaceutical Sciences ., 8:135-141.
Adebayo, J. O.; Adewum, O. S.; Baruwa , S. T.; Balogun, E. A.; Malomo, S. O.; Olatun ji, L. A. and Salodoye , A. O. (2016). “Effects of Polyphenols of Cocos Nucifera Husk Fibre on Selected Indices of Cardiovascular Diseases in Mice.” Tang (Humanitas Medicine )., 6:1-8.
Afroz ,R. E . M .; Tanvir, N . K .; Sabir Hossain, Md .; Nadia. A. and Ibrahim, K. S. G. Md. (2016) . “Sundarban Honey Confers Protection against Isoproterenol-Induced Myocardial Infarction in Wistar Rats.” BioMed Research International., 2016:1-10.
Allan, C. and Dawson, J. G. (1979). “Enzymatic Assay of Total Cholesterol Involving Chemical or Enzymatic Hydrolysis-a Comparison of Methods.” Clin. Cem., 25: 976–84.
Bishop, M. L.; Fody, E. P. and Schoeff, L. (2005). “Clinical Chemistry:Principles, Procedures, Correlations.” 5th Edn. eds.Lippincott Williams and Wilkins.Philadelphia., 200–202,285,290, 370,372,503–508.
Dang, G. K.; Parekar, R. R.; Kamat, S. K.; Scindia, A. M. and Rege, N. N. (2011). “AntiinFlammatory Activity of Phyllanthus Emblica, Plumbago Zeylanica and Cyperus Rotundus in Acute Models of Inflammation.” Phytother. Res., 25:904–908.
Duarte, M. M.; Rocha, J. B.; Moresco, R. N.; Duarte, T.; Da Cruz, I. B.; Loro, V. L. and Schetinger, M. R. (2009). “Association between Ischemia-Modified Albumin, Lipids and Inflammation Biomarkers in Patients with Hypercholesterolemia.” Clinical Biochemistry., 42: 666–71.
Friedwald, W. T.; Levy, R. I. and Fredrickson, D. S. (1972). “Estimation of Concentration of LDL-C in Plasma without the Use of Preparative Ultracentrifuge.” Clinical Chemistry 18: 499–502.
Graham, I.; Atar, D.; Borch-Johnsen, K.; Boysen, G.; Burell, G.; Cifkova, R.; Dallongeville, J.; De Backer, G.; Ebrahim, S.; Gjelsvik, B .(2007). “European Guidelines on Cardiovascular Disease Prevention in Clinical Practice.” Heart J ., 28: 2375–414.
Parikh, H.; Tripathi, C. B.; Shah, P.; Pharm, V.; Ghori. M. and Goyal, R. K. (2015). “Investigation of the Cardioprotective Effects of Crataegus Oxycantha and Its Molecular Mechanism.” Curr Res Cardiol., 2:161-167.
Heibashy, M. (2005). “Effect of Vanadium and Taurine on Lipid Profile and Some Parameters Indicative of Myocardial Status in Male Rats with Streptozotocin-Induced Diabetes.” J Egypt Ger Soc Zoology., 47: 117–38.
Jagadeesh, G. S.; Meeran, M. F. N. and Selvaraj, P. (2016). “Activation of β1-Adrenoceptor Triggers Oxidative Stress Mediated Myocardial Membrane Destabilization in Isoproterenol Induced Myocardial Infarcted Rats: 7-Hydroxycoumarin and Its Counter Action.” European Journal of Pharmacology., 777: 70–77.
Jahan, N.; Rahman, K. U. and Ali, S. (2012). “Cardioprotective and Antilipidemic Potential of Cyperus Rotundus in Chemically Induced Cardiotoxicity.” International Journal of Agriculture & Biology., 14: 989‒992.
Kaplan, L. A., Pesce, A. J. and Kazmierczak, S. C. (2003). “Clinical Chemistry: Theory, Analysis, Correlation.” 4th Edn. Eds. Mosby. London., 570–575,627.
Kesarwani, N. and Azmi, L . (2014). “Evaluation of Cardioprotective Effect of Tinospora Cordifolia against Isoprenaline Induced Myocardial Infraction in Rats.” International Journal of Current Microbiology and Applied Sciences., 5: 543–55.
Krushna, G.; Kareem, M. A. and Devi, K. L. (2009). “Antidyslipidaemic Effect of Aegle Marmelos Linn. Fruit on Isoproterenol Induced Myocardial Injury in Rats.” . Internet J Pharmacol., 6:2.
Kumar, S. R. and Kumar, S. A. (2013). “Cardio Protective Effect of Caesalpinia Crista Linn . on Isoproterenol Induced Myocardial Necrosis in Rats .” IJRPS., 3: 119–30.
Li, F.; Obrosova, I. G.; Abatan, O.; Tian, D.; Larkin, D.; Stuenkel, E. L. and Stevens, M. J. (2005). “Taurine Replacement Attenuates Hyperalgesia and Abnormal Calcium Signaling in Sensory Neurons of STZ-D Rats.” Am J Physiol Endocrinol Metab., 288: E29–36.
Lopes-Virella, M. F. (1977). “Cholesterol Determination in Highdensity Lipoproteins Separated by Three Different Methods.” Clin. Chem., 23: 882–84.
Lourenço, R. and Camilo, M. E. (2002). “Taurine: A Conditionally Essential Amino Acid in Humans? An Overview in Health and Disease.” Nutr. Hosp., 17: 262 – 70.
McCullough, M. L.; Peterson, J. J.; Patel, R.; Jacques, P. F.; Shah, R. and Dwyer, J. T. (2012(. “Flavonoid Intake and Cardiovascular Disease Mortality in a Prospective Cohort of US Adults.” The American Journal of Clinical Nutrition., 95: 454–64.
Murakami, S. Kondo-Ohta, Y. and Tomisawa, K. (1999). “Improvement in Cholesterol Metabolism in Mice given Chronic Treatment of Taurine and Fed a High-Fat Diet.” Life Sci., 64: 83–91.
Nordestgaard, B. G.; Chapman, M. J.; Ray, K.; Borén, J.; Andreotti, F.; Watts, G. F. and Tybjærg-Hansen, A. (2010). “Lipoprotein (a) as a Cardiovascular Risk Factor: Current Status.” European Heart Journal., 31: 2844–53.
Pop-Busui, R.; Sullivan, K. A.; Van, H. C.; Bayer, L.; Cao, X.; Towns, R. and Stevens, M. J. (2001). “Depletion of Taurine in Experimental Diabetic Neuropathy: Implications for Nerve Metabolic, Vascular, and Functional Deficits.” Exp Neurol., 168: 259–72.
Schaffer, S.; Azuma, J. and Mozaffari, M. ( 2009). “Role of Antioxidant Activity of Taurine in Diabetes.” Can J Physiol Pharmacol., 87: 91–99.
Shivakumar, S. I.; Suresh, H. M.; Hallikeri, C. S.; Hatapakki, B. C., Handiganur, J. S.; Sankh, K. and Shivakumar, B. (2009). “Anticonvulsant Effect of Cyperus Rotundus Linn. Rhizomes in Rats.” J. Nat. Rem., 9: 192–96.
Sudhakumari., Anil Kumar, H. V.; Javed, A.; Jaiswal, M. and Talkad, M. S. (2012). “Cardioprotective Effects in Methanolic Extract of Evolvulus Alsinoides Linn on Isoproterenol – Induced Myocardial Infraction in Albino Rats .” International Journal of Basic Medical Sciences and Pharmacy ( IJBMSP)., 2:53-57.
Sunmonu, T. O. and Afolayan, A. J. (2010) . “Protective Effect of Artemisia Afra Jacq. on Isoproterenol-Induced Myocardial Injury in Wistar Rats.” Food Chem, Toxicol., 48: 1969–1972.
Takeda, S.; Yamashita, A.; Maeda, K. and Meada, Y. (2003). “Structure of the Core Domain of Human Cardiac Troponin in the Ca (2+)-Saturated Form.” Nature., 424: 35–41.
Tchernof, A. and Després, J. P. (2013). “Pathophysiology of Human Visceral Obesity: An Update.” Physiological Reviews., 93: 359–404.
Tietz, N. W.; Burtis , C. A.; Ashwood, E. R. and Saunder, W. B. (1999). “Text Book of Clinical Chemistry.” 3rd Ed., 809–57.
Torres, N.; Torre-Villalvazo, I. and Tovar, A. R. (2006). “Regulation of Lipid Metabolism by Soy Protein and Its Implication in Diseases Mediated by Lipid Disorders.” The Journal of Nutritional Biochemistry., 17: 365–373.
Trachtman, H.; Del Pizzo, R. and Sturman, J. A. (1990). “Taurine and Osmoregulation III. Taurine Deficiency Protects against Cerebral Edema during Acute Hyponatremia.” Pediatr Res., 27: 85–88.
Upaganlawar, A.; Gandhi, H. and Balaraman, R. (2011). “Isoproterenol Induced Myocardial Infraction : Protective Role of Natural Products.” Journal of Pharmacology and Toxicology., 6: 1–17.
Published
2019-04-16
Section
Articles