Spcifies DNA fingerprint to Tilapia( Cichlidae) using RAPD-PCR in the Euphrates river in the Al-Muthann governorates

  • Marwa Qassim Animal Resource dep., Agriculture Coll., Al-Muthanna University
  • Massa’ad Taha Yassin Al-kafaji, Animal Resource dep., Agriculture Coll., Al-Muthanna University
  • Mustafa Sami Al- Fadag Animal Resource dep., Agriculture Coll., Al-Muthanna University
Keywords: DNA

Abstract

The DNA fingerprint of five population of tilapia species belonging to three species of tilapia  Oreochromis.niloticus, Oreochromis.aureus and Tilapia.zilli were collected from 90.  (10 from each of the three species)  samples of the Euphrates River passage in Muthanna Governorat e and the Shatt al-Arab River in Basra Governorate, southern Iraq. Using samples from each population were examined by RAPD-PCR technique using seven primer  (OPA08, OPA10, OPA13, OPA17, . OPA19, OPB09, OPC02) The results of this experiment revealed that the 7 primers produced a total of 106 easily scorable RAPD bands ranged in molecular size from approximately 46 to 2844 bp. DNA fingerprints of fish populations reach genetic relationships between them by drawing Dendrogram. Dendrogram is characterized by a high genetic similarity between two different population taken from two different T.zill environments, with a 0.76 ratio. Genetically similarity between two different population taken from two different environments of type O.aureus (0.50), while the results showed a high genetic similarity between O.niloticus , T.zilli. The genetic similarity between O.niloticus and O.aureus of the same family was 17.4 and there was little genetic similarity between O.aureus and T.zilli. The randomized RAPD-PCR method was able to differentiate between the five populations and reached the DNA signature of the species Three under environmental conditions in southern Iraq in this study had provided a baseline for future genetic improvement and monitoring of the gene pools of tilapia stocks

References

Abban, E.K., 1988. Taxonomy and biochemical genetics of some African freshwater fish species. University of Wales, U.K. 272 p Bielawski, J.p. and Pumo, D.E. (1997) Randomly amplified poly- morphic DNA(RAPD) analysis of Atlantic Coast striped bass. Heredity 78: 32-40
Elghobashy , A. A. Ramadan I. H. Ibrahem .2005phylogenetic relationships for some tilapia species using electrophoresis . The Egyptian Society of Experimental Biology . Egypt. J. Exp. Biol. (Zool.), 1: 57 – 63
Mutlak, F.M. and Al-Faisal, A.J. 2009. A new record of two exotic cichlids fish Oreochromis aureus (Steindacher, 1864) and Tilapia zilli (Gervais, 1848) fromsouth of the main outfall drain in Basrah city. Mesop. J. Mar. Sci., 24(2): 160-170
Mustafa Sami Faddagh,2014. Usage of new decamer primer MBl-9 to discriminate
six Barbus fish species(Cyprinidae: Teleostei) in Iraqi freshwaters. Dept. Aquaculture
and Marine Fisheries, Marine science Center, University of Basrah. ASccepted 22-10-2014
Kornfield , I. L., Ritte, U., Richler , C. and Wahrman , j. 1979 Biochemical and cytological differentiation among cichlid fishes of the Sea of Galilee. Evolution, 33, 1—14
Williams, J.G.k., Kubelik, A.R., Livak, K.J., rafalski, J.A. and Tingey, S.V. (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 18 (22):6531-6535.
Published
2019-04-17
Section
Articles