Study of the POU1f1 gene polymorphisms were associated with chicken growth traitsof Ross 308 broilers

  • Ali H. Abdulwahid
  • Ali A.Abdulkareem
  • Abdullah Hameed Salim
Keywords: traitsof Ross 308 broilers


This experiment was conducted in the poultry field of the Department of Livestock at the College of Agriculture and Marshlands /University of Thi-Qar for 42 days from 13/11 to 24/12/2020. The experiment included raising 100 broiler chickens of Ross 308 strain. The aim of this study was to detect the polymorphism of the PIT-1 gene in exon 6 of ROSS 308 broilers and their relationship to production performance with some biochemical blood traits. The PIT-1 gene is a transcription factor specific to the anterior lobe of the pituitary gland, and is involved in pituitary cell differentiation and production by mediating transcription of Growth hormone (GH) and Prolactin (PRL) and thyroid hormone (TSH-β) stimulation which make it an important gene for its effect on the productive traits in broilers. DNA was extracted from 100 birds, and the polymerase chain reaction and the Sequencing technology of Nitrogen Bases were used and the detection of genetic mutations occurring in them, and the results showed the presence of a point mutation (C1669T) in three genetic polymorphisms, namely CC , CT, and TT. There were highly significant differences (P>0.01) in the distribution ratios of genotypes, where the CC genotype scored the highest (82%), followed by the CT genotype(12%), and then the TT genotype(6%), and that the allelic frequency of the C allele is superior to that of the T allele. where they were 0.88 and. 0.12, respectively, and it was found that there was a significant difference (P<0.05) for the genotypes of the mutation (C1669T) on carcass weight, Dressing ratio, week body weight, relative breast weight, as well as serum total protein concentration at 42 days of age.



- Liu K. Muse SV (2005). PowerMarker: an integrated analysis environment for genetic marker analysis, Bioinformatics, 21 (9): 2128 2129

-Kulibaba,R. A.and Tereshchenko (2015). Polymorphism of growth hormone, growth hormone receptor, prolactin and prolactin receptor genes in connection with egg production in Poltava clay chicken. Sci. 62, 1584—1595

-Harvey, S., Johnson, C. D. M., Sharma, P., Sanders, E. J., & Hull, K. L. (1998). Growth hormone: a paracrine growth factor in embryonic development?. Comparative Biochemistry and Physiology Part C: Pharmacology, Toxicology and Endocrinology, 119(3), 305-315.

-Pfaffle C. Kim, R. W.,., Blankenstein, O., & Kentrup, H. (1999). GH transcription factors. Journal of pediatric endocrinology & metabolism: JPEM, 12, 311-317

-Wegner, M., Drolet, D. W., & Rosenfeld, M. G. (1993). POU-domain proteins: structure and function of developmental regulators. Current opinion in cell biology, 5(3), 488-498

- Liu, Lv, J., P., Gao, B., Wang, Y., Wang, Z., Chen, P., & Li, J. (2014). Transcriptome analysis of the Portunus trituberculatus: de novo assembly, growth-related gene identification and marker discovery. PLoS one, 9(4), e94055.‏

-Sambrook, J. (2001). Preparation and analysis of Eukaryotic Genome DNA. Molecular cloning: a laboratory manual, 1, 6-1

- Duncan, D.D. (1955). Multiple range and multiple F-test Biometrics,11:1-42

-Nguyen thi . A., Hung, L. T., Lan, L. T. T., Phong, N. H., Loc, H. T., Anh, L. H.,. T. (2021). The Association between POU1F1 Gene Polymorphisms and Growth as well as Carcass Traits of Noi Native Chickens. Journal of Advanced Veterinary Research, 11(1), 36-40 .‏

-Nie, Q., Fang, M., Xie, L., Zhou, M., Liang, Z., Luo, Z., ... & Zhang, X. (2008). The PIT1 gene polymorphisms were associated with chicken growth traits. BMC genetics, 9(1), 1-5

-Rodbari, Z., Alipanah , M., Seyedabadi, H. R., & Amirinia, C. (2011). Identification of a single nucleotide polymorphism of the pituitary-specific transcriptional factor 1 (Pit 1) gene and its association with body composition trait in Iranian commercial broiler line. African Journal of Biotechnology, 10(60), 12979-12983.‏

-Bhattacharya, T. K., Chatterjee, R. N., & Priyanka, M. (2012). Polymorphisms of Pit-1 gene and its association with growth traits in chicken. Poultry Science, 91(5), 1057-1064

‏13- AL-kreem, I. Q., Muhammad, M. F., & AL-Anbari, N. N. (2017) Association of Growth hormone gene polymorphisms (T3199C) SNP on productive and physiological performance in Hubbard broiler chicken Journal of Kerbala for Agricultural Sciences, 4(3), 25-36

-AL-Anbari(2019). Polymorphism of PIT 1 gene in broiler ROSS 308 hybrid and its association on some productive performance body scales. Toxicology and Endocrinology, 119(3), 305-315.

-Manjula, P., Choi, N., Seo, D., & Lee, J. H. (2018). POU class 1 homeobox 1 gene polymorphisms associated with growth traits in Korean native chicken. Asian-Australasian journal of animal sciences, 31(5), 643–649.

Jwar, A. S., & Abdul-Wahid, M. S. (2018). Effect of different pollen grain source and chitosan on some physiological and enzymatic characteristics of data palm Phoenix dactylifera L. fruits cv. Alshwethey. Basrah Journal For Date Palm Research, 17(1-2).

Abdul-wahid, M. S., & Jawar, A. (2019). Response of Some chemical, physical and productivity propertiesto date palmPhoenix dactylifera L. cultivation sites. University of Thi-Qar Journal of agricultural research, 8(1).

Abdul-wahid, M. S. (2019). Effect of Spray by Chelated Iron in physical and Chemical Characteristics of Iraqi Banana Musa Paradisica L. Var Sapientum (L.) Oktze Cultivation South Iraq. University of Thi-Qar Journal of agricultural research, 8(1).

-Jwar, A. S., & Neghamish, R. G. (2021). Effect of levels organic fertilizers and kinds of Bio-feritilizers in characterical of two classes of Okra plant (Abelmoschus esculentus L.) growning in the plastic house. University of Thi-Qar Journal of agricultural research, 10(1), 54-69.‏

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