Study of the POU1f1 gene polymorphisms were associated with chicken growth traitsof Ross 308 broilers
This experiment was conducted in the poultry field of the Department of Livestock at the College of Agriculture and Marshlands /University of Thi-Qar for 42 days from 13/11 to 24/12/2020. The experiment included raising 100 broiler chickens of Ross 308 strain. The aim of this study was to detect the polymorphism of the PIT-1 gene in exon 6 of ROSS 308 broilers and their relationship to production performance with some biochemical blood traits. The PIT-1 gene is a transcription factor specific to the anterior lobe of the pituitary gland, and is involved in pituitary cell differentiation and production by mediating transcription of Growth hormone (GH) and Prolactin (PRL) and thyroid hormone (TSH-β) stimulation which make it an important gene for its effect on the productive traits in broilers. DNA was extracted from 100 birds, and the polymerase chain reaction and the Sequencing technology of Nitrogen Bases were used and the detection of genetic mutations occurring in them, and the results showed the presence of a point mutation (C1669T) in three genetic polymorphisms, namely CC , CT, and TT. There were highly significant differences (P>0.01) in the distribution ratios of genotypes, where the CC genotype scored the highest (82%), followed by the CT genotype(12%), and then the TT genotype(6%), and that the allelic frequency of the C allele is superior to that of the T allele. where they were 0.88 and. 0.12, respectively, and it was found that there was a significant difference (P<0.05) for the genotypes of the mutation (C1669T) on carcass weight, Dressing ratio, week body weight, relative breast weight, as well as serum total protein concentration at 42 days of age.
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